A soluble enzyme system which catalyzes the aerobic oxidation of reduced pyridine nucleotides in wheat germ has previously been described (1). Peroxidase was shown to be a component of this system. Later, Humphreys (2) demonstrated that Mn+ and an unidentified cofactor were required for aerobic oxidation of TPNH’ in crude preparations of malic enzyme from wheat germ. Although no attempt was mad...

Peroxidase enzyme plays an important role in pharmaceutical, Industrial and various research laboratories. This enzyme can be purified by various different method. Our research trend was to purify the enzyme in a convenient and easy way. For this purpose a new affinity column was prepare by immobilizing PABA to N-HydroxySuccinamaideagarose gel. The presence of peroxidase was investigated on var...

Chance (1) has proposed that the peroxidase-catalyzed oxidation of diiydroxyfumaric acid proceeds in two steps. The first step is the oxidation of the hydrogen donor by oxygen, the reaction being catalyzed by a manganese-activated peroxidase and accompanied by the production of hydrogen peroxide. The second step is the oxidation of additional hydrogen donor by hydrogen peroxide, this reaction a...

Endogenous peroxidase activity has been demonstrated in sections of rat liver fixed briefly by glutaraldehyde perfusion and incubated in Graham and Karnovsky's medium for cytochemical demonstration of peroxidase activity (29). In 25-40% of sinusoidal cells, an electron-opaque reaction product is localized in segments of the endoplasmic reticulum, including the perinuclear cisternae, a few Golgi...

Iodination within the thyroid follicle is intimately associated with a thyroid peroxidase. In order to locate the in vivo site of iodination, the initial cytochemical appearance of this enzyme has been determined in fetal rat thyroid and its presence correlated with the onset of iodinated thyroglobulin synthesis. Peroxidase first appears in follicular cells during the 18th day of gestation. It ...

The endogenous defenses of the mouse heart against reactive oxygen metabolites were investigated. The activities of three enzymes capable of detoxifying activated oxygen were determined in both the heart and liver; cardiac muscle contains 150 times less catalase and nearly four times less superoxide dismutase than liver. Glutathione peroxidase activities were, however, similar to the two tissue...

The fine structural characteristics and phagocytic properties of peroxidase-positive and peroxidase-negative cells in rat hepatic sinusoids were investigated. Cells with a positive peroxidase reaction in the endoplasmic reticulum and the nuclear envelope make up approximately 40% of cells in rat hepatic sinusoids and have abundant cytoplasm containing numerous granules and vacuoles, and occasio...

Secretion of peroxidase by rat lacrimal glands is generally acknowledged to be greater in juvenile rats than in adults. However, this phenomenon has not been so well documented in lacrimal glands as other age-related changes have been. Therefore, we studied lacrimal protein and peroxidase secretion in response to muscarinic cholinergic and alpha-adrenergic stimulation of glands from 35- to 90-d...

A biotin-tyramine conjugate (1) was found to covalently cross-link with peroxidase antibody 7G12 upon the catalytic oxidation of the tyramine moiety in the presence of hydrogen peroxide (H2O2). On the basis of this observation, a novel strategy was developed to select mutants of 7G12 Fab with enhanced peroxidase activity from a library of phage displayed antibodies. In such a selection, tyramin...

The white rot fungus Phanerochaete chrysosporium has significant pollutant-degrading capabilities with its oxidoreductases. The lignin-degrading enzyme system of this fungus also allows the breakdown of different organic pollutants (xenobiotics). The most significant enzymes in these processes are the lignin peroxidase and the manganese peroxidase. In this study, Phanerochaete chrysosporium str...