نتایج جستجو برای: pet22b expression vector
تعداد نتایج: 1048484 فیلتر نتایج به سال:
objective: despite using the bacille calmette guerin (bcg) vaccine, tuberculosis (tb) is still a worldwide disease that kills 2-3 million people each year. developing a new and more effective vaccine is one way to possibly reduce the morbidity and mortality of tb. the mtb72f vaccine is one of the important subunit vaccines applied in human clinical trials. in this study, we have constructed an ...
Background: Human granulocyte colony-stimulating factor (hG-CSF) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. Transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other a...
Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...
Introduction Phytase is an enzyme that has the ability to break down phytic acid into myoinositol and mineral phosphate, and widely uses as an additive in animal foods. The aim of this study was to achieve a high level of bacterial phytase expression in PET26b expression host. Materials and Methods To generate the recombinant phytase enzyme, the target gene was introduced into the expression ...
سابقه و هدف: با توجه به اهمیت توالی ژن و کدونهای مربوط به هر اسید آمینه در میزبان بیان کننده پروتئین، همچنین به منظور تولید احتمالی واکسن بر علیه انفلوانزا a، یکی از عوامل اصلی مرگ ومیر سالیانه در جهان، این تحقیق انجام گرفته است. پروتئین m2 حفاظت شده است و بعد از تغییر در توالی آن به نظر میرسد هدف مناسبی برای تولید واکسن انفلوانزا در نظر گرفته شود. روش بررسی: تحقیق به روش تجربی انجام شد. توالی ...
Antifungal agents are causing different problems in the agriculture industry. Plants are using various defense mechanisms for resistance against fungal pathogens. Some examples of these mechanisms are making physical barriers, producing chemical components and pathogenesis-related proteins such as lipid transfer protein (LTP) and Osmotin which can inhibit the growth of fungi at micro-molar conc...
Background: Bovine follicle stimulating hormone (bFSH) belongs to the family of glycoprotein hormones that are released from the pituitary gland or the placenta. This hormone is a heterodimer consisting of common α- and specific β-subunit. Bovine FSH is used for reproductive technology such as superovulation in farm animals and polycystic ovary syndrome treatment. Also this hormone is produced ...
objective: the secreted aspartic proteinases (sap2) of candida albicans has prominent role on candida adherence, invasion, and pathogenicity. the aim of this study was cloning, expression and characterizing of sap2 enzyme. also in this study for the first time, the expression system p. pasturis was used for expressing the recombinant protein. materials and methods: c. albicans sap2 gene was am...
expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...
objectives: in present study we aimed to clone the lux i gene encoding n-acyl-homoserine synthase detected in biofilm forming clinical isolates of acinetobacter baumannii and study its expression in escherichia coli transformants. materials and methods: four a. baumannii hospital strains which demonstrated strong biofilm activity were selected in this investigation. the presence of lux i gene w...
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