A novel phytase with a molecular mass of 14 kDa was isolated from fresh fruiting bodies of the common edible mushroom Volvariella volvacea (Straw mushroom). The isolation procedure involved successive chromatography on DEAE-cellulose, CM-cellulose, Affi-gel blue gel, Q-Sepharose and Superdex-75. The enzyme was a monomeric protein and was unadsorbed on DEAE-cellulose, CM-cellulose and Affi-gel b...

A Gram-stain-negative, cream-pigmented, motile, extremely halophilic archaeon, designated strain IC38(T), was isolated from a saline mud sample taken from a hypersaline lake, Aran-Bidgol, in Iran. The strain required at least 2.5 M NaCl for growth. However, MgCl2 was not required. Optimal growth occurred with 4.3 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and ...

Phosphatidylethanolamine (once given the trivial name ‘cephalin’) is usually the second most abundant phospholipid in animal and plant lipids and it is frequently the main lipid component of microbial membranes. It can amount to 20% of liver phospholipids and as much as 45% of those of brain; higher proportions are found in mitochondria than in other organelles. As such, it is obviously a key b...

Phosphatidylethanolamine (once given the trivial name ‘cephalin’) is usually the second most abundant phospholipid in animal and plant lipids and it is frequently the main lipid component of microbial membranes. It can amount to 20% of liver phospholipids and as much as 45% of those of brain; higher proportions are found in mitochondria than in other organelles. As such, it is obviously a key b...

BACKGROUND Arterial puncture can be a painful procedure for many patients. This study investigates whether precooling of a puncture site with ice can reduce the pain associated with arterial puncture. METHODS This was a stratified randomized controlled trial of a convenience sample of out-patients with a physician order for an arterial blood gas (ABG) test. The intervention group had a plasti...

Three components, SF-973 A, B, and C were obtained from a Streptomyces olivaceus SF-973. SF-973 B catalyzed the converison of inactive SF-973 C (dihydroabikoviromycin) to bioactive SF-973 A (abikoviromycin). Purification of SF-973 B was conducted by sequential chromatographies on columns of DEAE-cellulose, Sephadex G-75, and Sephadex G-100. The enzyme SF-973 B was most active at around pH 7 and...

A thermophilic protease-secreting bacterial isolate, TPNK-3, from Kiteezi landfill, is an aerobic Gram-positive spore-forming bacterium with rod-shaped cells (3.28 μm long and 0.45 μm wide). Optimal growth was observed at 55°C and pH of 7.0, and the isolate tolerates up to 5% (w/v) NaCl, exhibits extracellular amylolytic, cellulolytic and caseinolytic activities, utilizes a range of carbon and ...

A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075T, was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gram-negative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 deg...

A simple, sensitive, and inexpensive high-performance liquid-chromatographic method has been developed for simultaneous determination of hydrochlorothiazide and candesartan cilexetil in pharmaceutical formulations. Chromatographic separation was achieved on a Phenyl-2 column with a 25:75:0.2 mixture of 0.02 M potassium dihydrogen phosphate, methanol, and triethylamine, final pH 6.0 ± 0.1, as mo...