Manganese peroxidase (MnP) is a major, extracellular component of the lignin-degrading system produced by the wood-rotting basidiomycetous fungus Phanerochaete chrysosporium. The transcription of MnP-encoding genes (mnps) in P. chrysosporium occurs as a secondary metabolic event, triggered by nutrient-nitrogen limitation. In addition, mnp expression occurs only under Mn2+ supplementation. Using...

Three fungal species were evaluated for their abilities to saccharify pure cellulose. The three species chosen represented three major wood-rot molds; brown rot (Gloeophyllum trabeum), white rot (Phanerochaete chrysosporium) and soft rot (Trichoderma reesei). After solid state fermentation of the fungi on the filter paper for four days, the saccharified cellulose was then fermented to ethanol b...

An indigenous locally isolated white rot fungal strain Phanerochaete chrysosporium IBL-03 was investigated for the hyper-production of ligninolytic enzymes from different agro-industrial wastes including wheat straw, rice straw, banana stalks, corncobs, corn stover, and sugarcane bagasse as substrate material in still culture fermentation technique. Screening experiments were performed at 30C f...

Paper and pulp industrial waste waters are a major environmental concern. As the treatment of these effluents is a major problem, exploring novel and feasible biotechnological interventions is very important. Utilization of white rot fungi as a means of biological treatment seems to be a viable option. The study was undertaken to use immobilization technique and Phanerochaete chrysosporium, a c...

The orotidylate decarboxylase (ODase) gene (ura1) from Schizophyllum commune was utilized as a reporter for studying Mn regulation of the manganese peroxidase (MnP) gene (mnp) from the lignin-degrading basidiomycete Phanerochaete chrysosporium. A 1,500-bp fragment of the mnp1 promoter was fused upstream of the coding region of the ODase gene in a plasmid (pAMO) containing the S. commune ade5 ge...

The electrophoretic behaviour of freshly harvested spores of Aspergillus niger, Phanerochaete chrysosporium and Geotrichum candidum was determined in solution at various pH values. Freshly harvested spores of all three species lacked positive mobility at low pH values, suggesting a preponderance of acidic surface groups. Spores of the ‘non-aggregating’ fungus, G. candidum, had a pH-mobility cur...

The continual expansion of urbanization and industrial activity has led to the accumulation of a large quantity of lignocellulosic residues throughout the world. In particular, large quantities of paper and bagasse are largely produced in Visakhapatnam. In this work we present the study of the degradability of these substrates with fungi. Three cultures of soil fungi were screened for their abi...

Thirtyfive siderophore producing fungi were categorized for their hydroxamate, catecholate or carboxylate nature by chemical and bioassays. Out of 35 fungi, 30 were hydroxamates and 5 showed carboxylate nature. However, none of the fungi produced catecholate type of siderophores. Eighteen out of 29 fungi were trihydroxamate and the rest 11 fungi were dihydroxamates. Twenty-three fungi were hexa...

Lignin peroxidases (LiP) of Phanerochaete chrysosporium are encoded by a family of six closely related genes. Five LiP genes have been localized to the same dimorphic chromosome. In this investigation, relative transcript levels of the LiP genes were determined. Transcripts of the LiPA, LiPB, and O282 genes were at similar levels in both carbon- and nitrogen-limited cultures. In contrast, trans...

Lignin biodegradation is catalyzed in part by ligninases, also known as lignin peroxidases (1-4). We have cloned and sequenced the gene encoding ligninase isozyme H8 from the white-rot fungus Phanerochaete chrysosporium. The gene is interrupted by eight introns, ranging in size from 49 to 69 bp, which are distributed throughout the gene. Putative transcriptional control signals (underlined) inc...