The Rieske nonheme iron aromatic ring-hydroxylating oxygenases (RHOs) NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1 have been implicated in the initial oxidation of high-molecular-weight (HMW) polycyclic aromatic hydrocarbons (PAHs), forming cis-dihydrodiols. To clarify how these two RHOs are functionally different with respect to the degradation of HMW PAHs, we investigated their subs...

The scale of the impact of petroleum products on the natural environment is still difficult to determine. This is why it was decided to conduct tests, under the conditions of a pot experiment, for the effects of diesel oil (0, 4, and 8 mL/kg dry matter of soil) on the yield of oat, content of macroelements, as well as the microbiological, biochemical, physicochemical and chemical properties of ...

In this study, the degradation potential of five polycyclic aromatic hydrocarbons (PAHs) by aerobic mixed bacterial cultures was investigated. Microorganisms were isolated from hydrocarbon contaminated soils of Shadegan wetland located in southwest of Iran. The degradation experiments were conducted in liquid cultures. PAH or PAHs concentration was 100 mg/L at the beginning of degradation exper...

Carcinogenic polynuclear hydrocarbons [7,12-dimethylbenzanthracene, 3-methylcholanthrene, and benzo(a)pyrene] were added to human skin fibroblast cell cultures. Only benzo(a)pyrene at 10 ¿ig/mlor above induced mixed-function hydroxylase activity, altered cell prolifer ation kinetics, and caused DNA damage as measured by altered grain count and bromodeoxyuridine incorporation. 3-Methylcholanthr...

A study for assessing the sources and fate of pollutants during their transport within urban catchment was carried out in the central Paris district, "Le Marais". 16 polycyclic aromatic hydrocarbons were assessed, according to the EPA list, in different types of runoff from a town centre and wet weather waste waters at the outlet of the catchment. PAHs were found predominantly in particulate ph...

Polycyclic aromatic hydrocarbons (PAHs) are regarded as key molecules in the astrochemical evolution of the interstellar medium, but the formation mechanism of even their simplest prototype-naphthalene (C(10)H(8))-has remained an open question. Here, we show in a combined crossed beam and theoretical study that naphthalene can be formed in the gas phase via a barrierless and exoergic reaction b...

The aim of this study was to compare the toxic effects of selected two- and three-ringed PAHs (naphthalene, phenanthrene, and anthracene) and their N-heterocyclic analogs with one (quinoline, acridine, and phenanthridine) or two (quinoxaline, phenazine, and 1,10-phenanthroline) nitrogen atoms on the survival and reproduction of Enchytraeus crypticus in artificial soil. Toxicity of compounds was...

Metabolism of the (+)-(3S,4S)and (-)-(3R,4R)-enantiomers of trnns-3,4-dihydroxy-3,4-dihydrobenz[a]anthracene has been examined with liver microsomes from control rats, as well as rats treated with 3-methylcholanthrene or phenobarbital and by a highly purified and reconstituted system containing cytochrome P-450, and epoxide hydrolase. In contrast to the metabolism of the related (-)-benzo[a]pyr...

A series of new heteroleptic and homoleptic Ru(II) complexes containing variously substituted bis(pyridyl)triazine ligands has been prepared and their absorption spectra, redox behaviour and luminescence properties (both in fluid solution at room temperature and in a rigid matrix at 77 K) have been investigated. For some compounds, X-ray structures have also been determined. The new bis(pyridyl...

The main goal of the study was the analysis of the parameters of wastewater generated during the ex situ underground coal gasification (UCG) experiments on lignite from Belchatow, and hard coal from Ziemowit and Bobrek coal mines, simulated in the ex situ reactor. The UCG wastewater may pose a potential threat to the groundwater since it contains high concentrations of inorganic (i.e., ammonia ...