Hamycin incorporated into liposomes containing phosphatidylcholine (SPC) and phosphatidic acid (PA) had reduced toxicity and an enhanced antifungal activity in experimental aspergillosis in balb/c mice. Incorporation of cholesterol into liposomes led to a dose dependent decrease in the toxicity of hamycin. The LD50 (mg/kg) of hamycin contained in SPC/cholesterol/PA (molar ratio 4:5:1) liposomes...

Adaptors appear to control clathrin-coat assembly by determining the site of lattice polymerization but the nucleating events that target soluble adaptors to an appropriate membrane are poorly understood. Using an in vitro model system that allows AP-2-containing clathrin coats to assemble on lysosomes, we show that adaptor recruitment and coat initiation requires phosphatidylinositol 4,5-bisph...

Fatty acyl coenzyme A-glycerol 3-phosphate, fatty acyl coenzyme A-lysophosphatidic acid, fatty acyl coenzyme A-monoglyceride, and fatty acyl coenzyme A-diglyceride acyltransferase activities were found in a particulate fraction of broken cell preparations of Escherichiu coli. The synthesis of phosphatidic acid and lysophosphatidic acid catalyzed by these enzymes was demonstrated to be dependent...

Opi1p, a transcription regulator of phospholipid metabolism in budding yeast, is retained in the endoplasmic reticulum by association with Scs2p. New research shows that binding of phosphatidic acid to Opi1p is a prerequisite for this targeting.