Extracts of vegetative cells of Blastocladiella emersonii contain 5% or less of the cyclic AMP phosphodiesterase activity in zoospore extracts. This difference in activity could be accounted for entirely by an increase in the differential rate of phosphodiesterase synthesis during sporulation, beginning after a lag period of about 60 min and extending for at least an additional 90 min into the ...

The 2',3'-cyclic nucleotide 3'-phosphodiesterase which hydrolyzes nucleoside 2',3'-cyclic phosphates (N greater than p) to nucleoside 2'-phosphates has been purified 16,000-fold to near homogeneity from wheat germ. The purified enzyme is a single polypeptide with a molecular weight of 23,000-24,000. It has a pH optimum of 7.0. The apparent Km values for A greater than p, G greater than p, C gre...

An enzyme that plays an important role in the repair of oxidative DNA damage is the 3'-phosphodiesterase. This activity, which repairs damaged DNA 3'-termini,can be detected using several available biochemical assays. We present a method to detect 3'-phosphodiesterase activity of renatured proteins immobilized in polyacrylamide gels. The model substrate, labeled with [alpha-32P]dCTP, contains 3...

Effects of divalent cations on the regulation of glucose transport and cAMP phosphodiesterase in isolated rat epididymal adipocytes were studied. EDTA (5 mM) moderately inhibited the binding of insulin to adipocytes in Krebs-Henseleit Hepes buffer. In the same buffer, A-23187 (an ionophore specific for divalent cations; 50 p ~ ) plus EDTA (5 mM) almost completely blocked the insulinor hydrogen ...

OBJECTIVE To examine the role of atrial natriuretic peptide (ANP) and cyclic GMP in the regulation of angiotensin converting enzyme (ACE) in cultured human endothelial cells. METHODS Cultured endothelial cells from human umbilical veins (HUVEC) were treated with ANP (0.3-30 nM), 8-Br-cGMP (1-100 microM), Rp-8-Br-PET-cGMPS (1 microM), or the phosphodiesterase inhibitors, zaprinast (10-100 micr...

Adenyl cyclase and cyclic 3’,5’-nucleotide phosphodiesterase were shown in homogenates of bovine corpora lutea. In order to determine the site of action of luteinizing hormone, the adenyl cyclase activity was assessed in the absence of phosphodiesterase activity. This was accomplished by using a high concentration of theophylline and short incubation periods. Under these conditions, luteinizing...

Cyclic nucleotide phosphodiesterase has been prepared from the liver, skeletal muscle, heart muscle, kidney, epididymal fat pad, and brain cortex of the rat. Kinetic analysis indicated that all of these tissues, with the exception of liver exhibit complex behavior indicative of multiple enzymatic activities. The phosphodiesterase activities of brain cortex, kidney, and adipose tissue have been ...

Photolyzed rhodopsin catalyzes the exchange of GTP for FDP bound to a protein in retinal rod outer segments. We previously proposed that the GTP complex of this protein regulates the cyclic GMP phosphodiesterase and that it may be the first amplified intermediate in visual excitation [Fung, B. K.-K. & Stryer, L. (1980) Proc. Natl. Acad. Sci. USA 77, 2500-2504]. We report here the identification...

The importance of adenosine 3’,5’-monophosphate (cyclic AMP) as a mediator for hormonal action needs no emphasis [I] . The level of CAMP is regulated by both the synthetic enzyme adenylate cyclase and the hydrolytic enzyme CAMP phosphodiesterase. While many useful methods have been developed for the biochemical assay of CAMP phosphodiesterase .activity [2-61, only a few of them have been adapte...

weight-gain and potency enhancing drugs are the most popular herbal products in iran which are easily available via the internet and through illegal markets. the content of some of these popularly purchased products were investigated for the presence of illegal substance like phosphodiesterase inhibitors (pdh-5) and corticosteroids by hplc method. for this study, ten samples of both kind of her...