Light and trace metals are critical growth factors for algae but how the interdependence of light quality and metal availability affects algal growth remains largely unknown. Our previous studies have demonstrated the importance of Ni and Fe on the growth of Trichodesmium and Symbiodinium, respectively, two important marine primary producers inhabiting environments with high light intensities. ...

E495 is the most abundant protease secreted by the Arctic sea-ice bacterium Pseudoalteromonas sp. SM495. As a thermolysin family metalloprotease, E495 was found to have multiple active forms in the culture of strain SM495. E495-M (containing only the catalytic domain) and E495-M-C1 (containing the catalytic domain and one PPC domain) were two stable mature forms, and E495-M-C1-C2 (containing th...

Abstract Phycocyanin, a phycobiliprotein, is one of the few natural blue pigments available as food colourant, and it largely used in industry. We have devised an innovative two-step extraction process which allowed to obtain bright phycocyanin crude extracts with high purity grade P (within 2.5 3.5) directly from fresh biomass Arthrospira platensis Gomont 1892 (commonly named Spirulina). found...

Abstract Arthrospira platensis is a cyanobacterium known for its widespread use as nutraceutical and food additive. Besides high protein content, this microorganism also endowed with several bioactivities related to health benefits in humans that make it candidate functional foods. These properties are strain culture condition dependent. We compared, terms of biomass productivity protein, C-phy...

Pseudanabaena limnetica, the cyanophycean microalga, like other members of Cyanophycea, is an excellent source pigments such as phycocyanin, proteins, carotenoids and polysaccharides. These strains also form a large proportion algal biomass. The P.limnetica strain can grow in extreme environmental conditions it grows well SW-BG 11 medium under laboratory conditions. In present investigation, th...

Phycocyanin (PC) is a bioactive compound that can function as an antioxidant, anti-inflammatory, immunomodulatory, and anti-cancer agent. It act potential material in preventing COVID-19 curing those suffering from it. Spirulina platensis (SP) one of the microalgae rich proteins PC. This study aimed to determine optimum PC extraction SP, using distilled water solvent through freezing-thawing pr...

C-Phycocyanin (C-Pc) is one of the major biliprotein pigments of unicellular cyanbacterium of Spirulina platenesis, it has nutritional, medicinal, and hepatoprotectant application. The growth and multiplication of human hepatoma cell lines (HepG2) under the effect of different concentrations of C-PC (0.8, 1.75, 3.5 and 7.0 microg/ml) against untreated cells as control for 24h were investigated....

Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model ...

We carried out the purification of C-phycocyanin and allophycocyanin from Spirulina platensis taking advantage of the adsorption properties of the expanded beds. Initially, phycobiliproteins were released from the microalga cells by osmotic shock. Next, phycocyanins were recovered by applying the centrifuged cell suspension directly to the anion exchanger Streamline-DEAE using expanded bed colu...

The level of glutamine synthetase activity in Agmenellum quadruplicatum strain PR-6 was dependent on the nitrogen source used for growth and on the nutritional status of the cells. During exponential growth, glutamine synthetase activity was low in cells grown on ammonia, urea, or nitrate. During the transition from nitrogen replete to nitrogen starved growth, glutamine synthetase activity bega...