F1 domain of F(1)F(o)-ATPase was initially believed to be strictly expressed in the mitochondrial membrane. Interestingly, recent reports have shown that the F1 complex can serve as a cell surface receptor for apparently unrelated ligands. Here we show for the first time the presence of the F(1)-ATPase at the cell surface of normal or cancerous colonic epithelial cells. Using surface plasmon re...

1. A mitochondrial fraction from beef heart (TU-particles) was shown to confer rutamycin sensitivity to added mitochondrial ATPase (F,). After sonic oscillation at an alkaline pH, the resulting particles (TUA-particles) no longer had this activity. A rutamycin sensitivity factor (FJ, purified from crude preparations of coupling factor 4, restored the capacity of the particles to confer rutamyci...

Introduction The aim was to determine the mitochondrial content, oxidative and nitrosative status in placentas from pregnant women who delivery newborns with alteration of intrauterine growth. Material methods Placentas were selected because classified as small for gestational age (SGA, lowest 10th percentile; n = 9), appropriate (AGA; 9) large (LGA, tallest 90th 9). In placenta tissue status, ...

Abstract The aim of this study was to evaluate the effect ozone treatment on mitochondrial energy metabolism in blueberry fruit during storage as well determine relationship between activity mitochondria and antioxidative properties ozonated fruit. Blueberry stored for 28 days at 4 °C daily with gaseous concentration 15 mg L −1 30 min, every 12 h storage. Research showed that characterized by h...

Mutations of mitochondrial DNA are associated with a wide spectrum of disorders, primarily affecting the central nervous system and muscle function. The specific consequences of mitochondrial DNA mutations for neuronal pathophysiology are not understood. In order to explore the impact of mitochondrial mutations on neuronal biochemistry and physiology, we have used fluorescence imaging technique...

The PINK1-Parkin pathway plays a critical role in mitochondrial quality control by selectively targeting damaged mitochondria for autophagy. In this issue, Tanaka et al. (2010. J. Cell Biol. doi: 10.1083/jcb.201007013) demonstrate that the AAA-type ATPase p97 acts downstream of PINK1 and Parkin to segregate fusion-incompetent mitochondria for turnover. p97 acts by targeting the mitochondrial fu...

Plants of genus Polygonatum (Ruscaceae) are widely used in traditional medicine to cure many diseases such as ageing, antioxidative action, insecticidal, antiherpetic, antinociception and aphrodisiac. A variety of phytoconstituents has been isolated from the Polygonatum species which include lignans, flavonoids, coumarins, steroids, terpenes, fatty acids and aliphatic long chain compounds. Anti...

Mitochondria from dormant spores of the fungus Botryodiplodia theobromae did not contain a functional oligomycinor dicyclohexylcarbodiimide-sensitive adenosine triphosphatase (ATPase); however, this enzyme activity was elaborated rapidly after 120 min of a 240-min germination sequence. The development of oligomycin-sensitive ATPase in germinating spores was abolished by cycloheximide but initia...

Earlier work has shown that mitochondrial proteins synthesized in the cytosol are initially made as larger precursors which are then transferred into the organelles and processed to their mature size in the absence of protein synthesis. It is now demonstrated that depletion of the mitochondrial matrix ATP in intact yeast spheroplasts by various combinations of inhibitors and mutations prevents ...