Extraction of RNA from plant tissue containing high levels of polyphenols and polysaccharides is tedious and difficult in grapes. Although several protocols have been published for plant RNA isolation, most have failed to yield high quality RNA in sufficient quantity from mature and diseased grape tissue. We describe a protocol for isolating intact and high quality RNA from various grape tissue...

the effects of sodium chloride (nacl) salinity (control; canal water with 0.4 ds m-1), 2.5 dsm-1, 5.0 ds m-1, 7.5 ds m-1 and 10.0 ds m-1, respectively) developed after 6 weeks of pruning (beginning of study) on plant growth, flowering and quality of three cut rose (rosa hybrida l.) cultivars viz. ‘kardinal’, ‘angelique’ and ‘gold medal’ were studied to achieve better management, quality product...

An experiment was conducted to evaluate the effect of different sowing time on seed yield and quality in under used medicinal plant babchi. The study was carried out for 12 months from January to December. Results showed that the seeds sown in November month recorded the maximum seed yield and quality parameters followed by October month than other months. November month recorded the maximum fi...

Isolation of high-quality RNA free of contaminants, such as polyphenols, proteins, plant secondary metabolites, and genomic DNA from plant tissues, is usually a challenging but crucial step for molecular analysis. We developed a novel protocol based on the cetyltrimethylammonium bromide method to isolate high-quality RNA from blackberry plant tissues, especially fruits. Most DNA was remove...

Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has h...

BACKGROUND Plant fungal pathogens play a crucial role in the profitability, quality and quantity of plant production. These phytopathogens are persistent in avoiding plant defences causing diseases and quality losses around the world that amount to billions of US dollars annually. To control the scourge of plant fungal diseases, farmers have used fungicides to manage the damage of plant pathoge...

UNLABELLED PREMISE OF THE STUDY To study gene expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNAlater. Obtaining sufficient and high-quality yield from variously preserved samples is essential to studies of com...

We compared plant diversity in four restored and four natural wetlands in the glaciated interior plains to evaluate how quickly plant biodiversity develops following cessation of agriculture and reintroduction of wetland hydrology. After approximately ten years, restored wetlands had plot and site richness comparable to natural wetlands; 6.2 + 0.5 species per plot and 33.8 + 2.3 species per sit...

Standardization of natural products is a complex task due to their heterogeneous composition, which is in the form of whole plant, plant part or extracts obtained thereo f. To ensure reproducible quality of herbal products, proper control of starting material is utmost essential. The first step towards ensuring quality of starting material is authentication. Thus, in recent years there has been...