The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...

Rapid plasma membrane resealing is essential for cellular survival. Earlier studies showed that plasma membrane repair requires Ca(2+)-dependent exocytosis of lysosomes and a rapid form of endocytosis that removes membrane lesions. However, the functional relationship between lysosomal exocytosis and the rapid endocytosis that follows membrane injury is unknown. In this study, we show that the ...

Pseudomonas aeruginosa is an opportunistic pathogen that is associated with hospital-acquired infections, ventilator-associated pneumonia, and morbidity of immunocompromised individuals. A subpopulation of P. aeruginosa encodes a protein, ExoU, which exhibits acute cytotoxicity. Toxicity is directly related to the phospholipase A2 activity of the protein after injection into the host cytoplasm ...

because of a paucity of information on the effect of satureja khuzistanica in male chickens, this study was undertaken to determine the influence of dietary s. khuzistanica powder (skp) on seminal characteristics and testes thiobarbituric acid reactive substances (tbars) content in iranian native breeder rooster. thirty-six 40-week-old roosters were randomly allotted to 3 equal groups and recei...

experiment was conducted to evaluate the effect of different levels of egg yolk for coating and storing spermatozoa. ejaculates were collected from four rams. in each session, second ejaculates (n=4) were collected in a tube containing 1 ml coating buffers which were prepared by 10, 15 or 20% egg yolk plus tris-fructose. samples were pooled, centrifuged and the supernatant removed in the labora...

polyethylene glycol (peg) is a hydrophilic polymer, known to be capable to fuse numerous single cells in vitro, to join the membranes of adjacent neurons and giant invertebrate axons, and to seal damaged neural membranes. the molecular mechanism of the action of peg is still unknown. it is believed that peg dehydrates membranes and enables their structural components to resolve and rearrange in...

Gel-filtered blood platelets exposed to complement proteins C5b-9 have previously been shown to undergo a reversible depolarization of membrane potential (Em) in the absence of lytic plasma membrane rupture. In this paper, we examine the mechanism by which C5b-9 damaged platelets restore their basal electrochemical state, despite increased ion conductance due to membrane insertion of these cyto...

introduction: gold standard for alveolar cleft bone grafting is autogenous bone. advantage of adding the growth factors to the bone is established, so adding prf to the autogenous bone may have beneficial effects. methods:  platelet-rich plasma admixed with autogenous bone and as a biologic membrane over the bone grafted alveolar cleft was used. results:  autogenous bone in all (four cases) was...

the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...

The aim of this study was to investigate the effects of catalase and pre-freezing equilibration during ram sperm cryopreservation on motility and membrane and acrosomal integrity of frozen-thawed semen, as well as conception rate following laparoscopic timedinsemination. Semen was collected from four mature Dorper rams, pooled and diluted in Tris egg-yolk extender basic solution (CON), or this ...