Plasmids maintain themselves in their bacterial host through several different mechanisms, one of which involves the synthesis of plasmid-encoded toxin and antitoxin proteins. When the plasmid is present, the antitoxin binds to and neutralizes the toxin. If a plasmid-free daughter cell arises, however, the labile antitoxin is degraded (and not replenished) and the toxin kills the cell from with...

INTRODUCTION AND DEFINITIONS ...................................... 361 SOME PROPERTIES OF BACTERIAL PLASMIDS .......................... 362 Chromosomal Integration ............... .................................... 363 Fertility Repression........................................................ 363 Incompatibility ............................................................. 364 Elimination ...

We have developed high-throughput microtitre plate-based assays for DNA gyrase and other DNA topoisomerases. These assays exploit the fact that negatively supercoiled plasmids form intermolecular triplexes more efficiently than when they are relaxed. Two assays are presented, one using capture of a plasmid containing a single triplex-forming sequence by an oligonucleotide tethered to the surfac...

Colistin resistance in Acinetobacter spp. is increasing, resulting potentially untreatable nosocomial infections. Plasmid-mediated colistin of particular concern due to its low fitness cost and potential transferability other bacterial strains species. This study investigated the mechanism a clinical nosocomialis isolate from Cape Town, South Africa. A colistin-resistant A. was identified blood...

In molecular biology for cloning experiments, plasmid, a circular molecule is used as vector (carrier) to insert a desired gene or DNA fragment by ligating into it after digestion of both with the compatible restriction enzymes. Then by transforming with a host (generally E. coli) strain of desired traits the screening for appropriate clone is done. A plasmid is having selectable marker in the ...

BACKGROUND Prior study indicates that abnormal protein expression and functional changes in the development and progression of colorectal cancer is related to gene expression. The aim of this study was to construct an interference plasmid targeting the Ep-CAM gene and to investigate its effects on the proliferation of colorectal cancer cells. METHODS In this study, HT-29 and HCT-116 colorecta...

Background: Crimean-Congo hemorrhagic fever virus (CCHFV) is a highly lethal that causes in humans and endemic many countries, including Iran. Therefore, fast, accurate, reliable diagnosis crucial for patient management outbreak control. Objectives: This study aims to optimize TaqMan multiplex real-time RT-PCR the rapid specific of CCHFV. Methods: In this study, L (NC_005301.3) S (NC_005302.1) ...

In previous studies, the present authors demonstrated that effective sensitization of ionizing radiation-induced death of tumor cells, including non-small cell lung cancer (NSCLC) cells, could be produced by oleanolic acid (OA), a pentacyclic triterpenoid present in plants. In the present study, it was investigated whether ursolic acid (UA), an isomer of OA, had also the capacity of sensitizing...

The aim of this study was to examine the effect of Annexin A1 (ANXA1) on the proliferation, migration and invasion of esophageal squamous cell carcinoma (ESCC) cells and its possible mechanisms of action. After constructing the ANXA1 overexpression plasmid, we transfected this plasmid and/or microRNA (miRNA)‑196a mimic into ESCC cells (Eca109 cell line). Methyl thiazolyl tetrazolium (MTT) assay...