Background: The utilization of methane for production of Poly-β-hydroxybutyrate (PHB) not only cuts the emissions of greenhouse gases but also greatly reduces PHB production cost. Objectives: The aim of this study was to determine the effects of gas-phase conditions on PHB production by Methylosinus trichosporium OB3b. Ma...

In our previous paper, we synthesized poly-3-hydroxybutyrate [P(3HB)] by using the water-organic solvent two-phase reaction system (TPRS), in which thiophenyl (R)-3-hydroxybutyrate [(R)-3HBTP] was used as a precursor of 3HBCoA. We have developed an improved TPRS for the chemo-enzymatic synthesis of polyhydroxyalkanoate (PHA). In this method, acetyl thioester of ethyl thioglycolate (AcETG) was u...

Novel biodegradable poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [PHBV]/graphene nanocomposites were prepared by solution casting. The thermal properties, crystallization behavior, microstructure, and fracture morphology of the composites were investigated. Scanning electron microscope (SEM) results show that graphene layers are homogeneously dispersed in the polymer matrix. X-ray diffraction (...

We have cloned and sequenced the 3-hydroxybutyrate dehydrogenase-encoding gene (bdhA) from Rhizobium (Sinorhizobium) meliloti. The gene has an open reading frame of 777 bp that encodes a polypeptide of 258 amino acid residues (molecular weight 27,177, pI 6.07). The R. meliloti Bdh protein exhibits features common to members of the short-chain alcohol dehydrogenase superfamily. bdhA is the first...