The structure of methionyl-tRNAfMet(f) formyltransferase from E. coli, a monomeric protein of 34 kDa, has previously been determined at 2.0 A resolution. In the present work, this enzyme was crystallized as a complex with its macromolecular product, the initiator formyl-methionyl-tRNAfMet(f) (25 kDa). Polyethylene glycol 5000 monomethylether was used as a precipitating agent. The crystals are o...

We describe the production of collagen fibre bundles through a multi-strand, semi-continuous extrusion process. Cross-linking using an EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide), NHS (N-hydroxysuccinimide) combination was considered. Atomic Force Microscopy (AFM) and Raman spectroscopy focused on how cross-linking affected the collagen fibrillar structure. In the cross-linked fibres, a...

Hamster sarcoma virus (HaSV), a ribonucleic acid tumor virus, pelleted from tissue culture fluid manifests type C morphology by electron microscopy. However, if virus is first concentrated by polyethylene glycol or ammonium sulfate followed by density gradient banding, the virus shows a dramatically atypical barred core structure, termed "theta particles." This structure suggests a condensation...

We have previously reported the first realization of an orthogonal ferroelectric bent-core SmAPF phase by directed design in mesogens with a single tricarbosilane-terminated alkoxy tail. Given the potentially useful electrooptic properties of this phase, including analog phase-only electrooptic index modulation with optical latching, we have been exploring its "structure space", searching for n...

INTRODUCTION Since many decades, polyethylene (PE) is one of the commodity polymers which has become ubiquitous because of its low price and good mechanical properties. Hence, the number of applications of the material is huge and many millions of tons are produced worldwide annually. However, PE has hardly played any role in the emerging field of nanotechnology so far. This is due to the probl...

Developing an appreciation for the mechanical behavior of polymers materials in a lecture mode can be challenging for students if they have not bad the benefit of a laboratory experience. We have developed a simp le demonstration of thermoplastic polymeric behav ior using low-density polyethylene bags. The demonstration illustrates strengthening, rate effects, and directionality of strengthenin...

Wang comments that the diffraction data for the structure of the A139T mutant of translocator protein TSPO from Rhodobacter sphaeroides should be used to 1.65 instead of 1.8 angstroms and that the density interpreted as porphyrin and monoolein is better fitted as polyethylene glycol. Although different practices of data processing exist, in this case they do not substantially influence the fina...