The pulp and paper industry is recognized as a well-established sector, which throughout its process, generates vast amount of waste streams with the capacity to be valorized. Typically, these residues are burned for energy purposes, but their use substrates biological processes could more efficient sustainable alternative. With this aim, it essential identify characterize each type determine b...

The superior biocompatibility and biodegradability of polyhydroxyalkanoates (PHAs) compared to man-made biopolymers such as polylactic acid promise huge potential in biomedical applications, especially tissue engineering (TE). Textile fiber-based TE scaffolds offer unique opportunities imitate the anisotropic, hierarchical, or strain-stiffening properties native tissues. A combination PHAs’ enh...

Microalgae biomass has been recently used as an inexpensive substrate for the industrial production of polyhydroxyalkanoates (PHAs). In this work, a dilute acid pretreatment using 0.3 N hydrochloric (HCl) was performed to extract reducing sugars from 10% (w/v) defatted Chlorella (DCB). The resulting HCl DCB hydrolysate renewable assess ability three bacterial strains, namely Bacillus megaterium...

The accumulation of polyhydroxyalkanoates (PHAs) by microorganisms usually occurs in response to environmental stress conditions. Therefore, it is advantageous choose two-step cultivation. first phase aimed at maximizing biomass production, and only the second phase, after setting suitable conditions, PHA production starts. aim this work was optimize composition minimal propagation medium used ...

The most promising drug delivery systems today are biodegradable microspheres and microcapsules. Microspheres can be injected subcutaneously or intramuscularly, adapted for oral administration or inhalation, and introduced into the bloodstream (Ueda 2003). The rapid development of microencapsulation has triggered an interest in biocompatible and biodegradable polyhydroxyalkanoates (PHAs), which...

Metabolically engineered Escherichia coli JM109 harboring plasmid pBPP1 and expressing the nonnatural BPEC pathway for synthesis of thermoplastic polyhydroxyalkanoates (PHA) and novel polythioesters (PTE) to provide suitable substrates of PHA synthase was investigated with respect to biotechnological production of poly(3-mercaptopropionate) [poly(3MP)]. Fed-batch fermentation processes were est...

This integrated study shows that waste glycerol can be bio-valorized by the fabrication of electrospun scaffolds for stem cells. Human mesenchymal stem cells (hMSC) provide an interesting model of regenerating cells because of their ability to differentiate into osteo-, chrondro-, adipo- and myogenic lineages. Moreover, hMSC have modulatory properties with potential on treatment of immunologic ...

3D printing represents a valuable alternative to traditional processing methods, clearly demonstrated by the promising results obtained in the manufacture of various products, such as scaffolds for regenerative medicine, artificial tissues and organs, electronics, components for the automotive industry, art objects and so on. This revolutionary technique showed unique capabilities for fabricati...

A new protein immobilization and purification system has been developed based on the use of polyhydroxyalkanoates (PHAs, or bioplastics), which are biodegradable polymers accumulated as reserve granules in the cytoplasm of certain bacteria. The N-terminal domain of the PhaF phasin (a PHA-granule-associated protein) from Pseudomonas putida GPo1 was used as a polypeptide tag (BioF) to anchor fusi...