The intended circular economy for plastics envisages that they will be partially replaced by bio-based polymers in the future. In this work, natural polyester polyhydroxybutyrate (PHB) was produced Azohydromonas lata using cheese whey (CW) as a low-cost substrate. Initially, CW evaluated sole carbon source PHB production; it found to efficient and comparable production with pure sugars, such sa...

Fibrils formed by human serum transferrin [(1-3 μM) apo-Tf, partially iron-saturated (Fe0.6 -Tf) and holo-Tf (Fe2 -Tf) forms], from dilute bicarbonate solutions, were deposited on formvar surfaces and studied by electron microscopy. We observed that possible bacterial contamination appears to give rise to long, pea-pod-like (PPL) structures for Fe2 -Tf, attributable to the formation of polyhydr...

It has been claimed that citrate synthase, aconitase and isocitrate dehydrogenase activities are non-functional in Bordetella pertussis and that this might explain why this bacterium's growth is sometimes associated with accumulation of polyhydroxybutyrate (PHB) and/or free fatty acids. However, the sequenced genome includes the entire citric acid pathway genes. Furthermore, these genes were ex...

Protein purification of recombinant proteins constitutes a significant cost of biomanufacturing and various efforts have been directed at developing more efficient purification methods. We describe a protein purification scheme wherein Ralstonia eutropha is used to produce its own "affinity matrix," thereby eliminating the need for external chromatographic purification steps. This approach is b...

In this study, polyhydroxybutyrate (PHB)--a biodegradable plastics material--was produced by activated sludge performing enhanced biological phosphorus removal (EBPR) in batch experiments under anaerobic, aerobic and anaerobic/aerobic conditions. Under anaerobic conditions, the maximum PHB content of the dry biomass was 28.8% by weight, while under aerobic or anaerobic/aerobic conditions, the m...

Industrial-scale production of biopolymers is restricted by its elevated production costs in comparison with those associated with synthetic (no-biodegradable and no-biocompatible) polymers. In this study we tested for the first time two low-cost carbon substrates (i.e. carob pulp and fique juice) for lab-scale production of polyhydroxyalkanoate (PHA) with Bacillus megaterium. PHA detection and...

The production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using industrial residues, crude glycerol from biodiesel synthesis and rice hull hydrolysate (RHH), as low-cost carbon sources was investigated. experiments were conducted shaking flasks at 30 °C 180 rpm up to 72 h. extraction PHA carried out sodium hypochlorite make its recovery more environmentally friendly avoiding organic...

Type II methanotrophs produce polyhydroxybutyrate (PHB), while Type I methanotrophs do not. A laboratory-scale fluidized bed reactor was initially inoculated with a Type II Methylocystis-like dominated culture. At elevated levels of dissolved oxygen (DO, 9 mg/L), pH of 6.2-6.5 with nitrate as the N-source, a Methylobacter-like Type I methanotroph became dominant within the biofilms which did no...

Gut microbiome shapes various aspects of a host's physiology, but these functions in aquatic animal hosts have yet to be fully investigated. The sea cucumber Apostichopus japonicus Selenka is one such example. The large growth gap in their body size has delayed the development of intensive aquaculture, nevertheless the species is in urgent need of conservation. To understand possible contributi...

Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by microbes to overcome environmental stress. Commercial production of PHAs is limited by the high cost of production compared to conventional plastics. Another hindrance is the brittle nature and low strength of polyhydroxybutyrate (PHB), the most widely studied PHA. The needs are to produce PHAs, which have better elastomeric pr...