Many mutation detection techniques rely upon recognition of mismatched base pairs in DNA hetero-duplexes. Potassium permanganate in combination with tetraethylammonium chloride (TEAC) is capable of chemically modifying mismatched thymidine residues. The DNA strand can then be cleaved at that point by treatment with piperidine. The reactivity of potassium permanganate (KMnO4) in TEAC toward mism...

for mistura carminativa, the two bottles being together on her table. As soon as she had done so she realized her mistake and vomited the medicine as far as she was able to do. Medical aid was at once called in and her stomach was immediately washed out with potassium permanganate solution. I saw her after the stomach had been washed out the first time and noticed typical symptoms of aconite po...

1. The isolation and properties of roseins I and II from the mycelium ofTrichothecium rogeum and of rosein III from the culture filtrate are described. 2. Rosein I, colourless tetrahedra, m.p. 2100, [oc]4112-5 in chloroform (c, 1) has the molecular formula C019H2603. It is a neutral ketone, which reacted with one equivalent of hot ethanolic alkali and yielded a lactone, i8orosein I, m.p. 144°, ...

The fixation of rat pancreas in potassium permanganate according to Luft (1) imparts a brown coloration and a strong ultraviolet absorption to the cytoplasm of the exocrine cells, but only a weak one to the nuclei. Within the latter an even paler zone surrounding the nucleoli can be observed. After the removal of the stain by means of oxalic acid, ultraviolet microscopy and the Feulgen technic ...

Analyses of Drosophila cells have revealed that RNA polymerase II is paused in a region 20 to 40 nucleotides downstream from the transcription start site of the hsp70 heat shock gene when the gene is not transcriptionally active. We have developed a cell-free system that reconstitutes this promoter-proximal pausing. The paused polymerase has been detected by monitoring the hyperreactivity of th...

Guanine-rich DNAs can fold into four-stranded structures that contain stacks of G-quartets. Bioinformatics studies have revealed that G-rich sequences with the potential to adopt these structures are unevenly distributed throughout genomes, and are especially found in gene promoter regions. With the exception of the single-stranded telomeric DNA, all genomic G-rich sequences will always be pres...

Frankia vesicles are differentiated during nitrogen starvation; they contain nitrogenase whether produced by free-living frankiae or by frankiae in actinorhizal root nodules. Vesicles are surrounded by envelopes of several monolayers of uncharacterized lipid. It has been suggested that the envelope limits diffusion of O2 into the vesicle cytoplasm, thereby preventing inactivation of nitrogenase...

gonococcus (see below). Neither local irrigation nor oral therapy producing antiseptics in the urine can affect organisms in the Littre gland. Intravenous therapy that aims to apply the antiseptic direct to the organisms in the glands through the blood stream is more rational, but in practice is unsatisfactory. Mercurochrome and acriflavine are not sufficiently specific to kill the organisms in...