Slices of mammary gland from lactating rats, incubated with acetate-1-(14)C or mevalonate-2-(14)C, synthesized cholesterol-(14)C. Within 2 min of an intravenous injection of 1 ml of a suspension of chylomicrons containing cholesterol-4-(14)C, mammary glands of lactating rats removed as much as 29% of the labeled cholesterol; those of 2-day-postlactating rats removed none. Rats were fed a diet c...

The objectives of this study were to measure the changes in protein expression of the mammary Ca2+-ATPases during the periparturient period and to determine whether Ca2+-ATPase protein expression in the mammary gland is related to milk fever (MF) development. Abundance of Ca2+-ATPase in mammary tissue and milk fat globule membranes was determined by Western blotting. The secretory pathway Ca2+-...

The term "deciduosis" is used to describe the severe pregnancy-associated occurrence of ectopic decidua with a usually asymptomatic course. We report on two cases of massive maternal intra-abdominal bleeding due to such symptomatic changes. The complications arose at different time points for the two cases: prepartum (26th week of pregnancy) or, respectively, - reported here for the first time ...

The objective of this study was to assess, in the context typical dairy farms western France, preventive effects prepartum diet acidification (AcD) and mineral intake during late gestation on incidence subclinical hypocalcemia (SHC) hypophosphatemia (SHP) after calving. We conducted a longitudinal that followed cohort 371 Holstein cows from 26 French farms; these, 235 (15 farms) were supplied w...

The study was conducted to evaluate the effect of vitamin E and zinc supplementation on energy metabolites, lipid peroxidation, and milk production in peripartum Sahiwal cows. For this, thirty-two pregnant dry Sahiwal cows were selected at sixty days prepartum and divided into four groups viz control, T1, T2, and T3 of eight each. Group T1 were supplemented with zinc at 60 ppm/d/cow, group T2 w...

Exposure to poor maternal nutrition around the time of conception results in an early prepartum activation of the fetal pituitary-adrenal axis and in increased adrenal growth and stress response after birth associated with epigenetic changes in a differentially methylated region (DMR) of adrenal IGF2/H19. We have determined the effects of maternal undernutrition during the periconceptional peri...

Prevalence of intramammary infections at prepartum and postpartum in primigravid heifers from five dairy herds located in the central dairy area of Argentina was determined. Mammary secretion samples from 140 heifers (560 mammary quarters) were obtained 14 days prior to the expected calving day and within 7 days after parturition and subjected to bacteriological analysis. No clinical mastitis c...