MOTIVATION EMAN is one of the most popular software packages for single particle reconstruction. But the particle clusters produced during its model refining stage are of low qualities. We attempt to refine the particle clusters by more accurately determining orientations of particles, and thereby achieving higher resolutions of consequent 3D structures. RESULTS A particle reclustering framew...

The Probabilistic Relevance Framework (PRF) is a formal framework for document retrieval, grounded in work done in the 1970–1980s, which led to the development of one of the most successful text-retrieval algorithms, BM25. In recent years, research in the PRF has yielded new retrieval models capable of taking into account document meta-data (especially structure and link-graph information). Aga...

BACKGROUND There is little evidence regarding the effectiveness of intervention methods in the treatment of zoster-related pain (ZAP) after the acute phase of zoster. Generally, if ZAP remains after more than 180 days from its onset, the likelihood of pain reduction is very low; this condition is considered as a "well established" post-herpetic neuralgia (PHN). Although the clinical efficacy of...

BACKGROUND Neuropathic pain (NP) is a major public health problem worldwide. Because of the unclear mechanism of NP, its treatment is one of the most difficult medical problems. As a targeted, noninvasive, safe therapy, pulsed radiofrequency (PRF) provides a new method for the treatment of NP; however, its effect on this treatment still lacks support from evidence-based medicine. OBJECTIVE To...

Hemifacial spasm is defined as unilateral, involuntary, irregular twitching of all or parts of the muscles innervated by facial nerves. Here, we present a case of recurrent hemifacial spasm after microvascular decompression (MVD) treated with pulsed radiofrequency (PRF) treatment with good results. A 35-year-old woman suffered from recurrent hemifacial spasm after MVD that was refractory to med...

We demonstrate amplification of picosecond laser pulses to 40?mJ at a 2?kHz pulse repetition frequency (PRF) from a two-stage cryogenic chirped-pulse Yb:YAG amplifier, composed of a regenerative amplifier (RGA) and a two-pass booster amplifier. The RGA produces 8.2mJ of energy at 2kHz PRF and 13.2mJ at 1kHz PRF with excellent energy stability (approximately 0.3% rms) and beam quality (M(2)<1.1)...

Diabetic wounds are serious complications caused by diabetes mellitus (DM), which further exacerbated angiogenesis disorders and prolonged inflammation. Injectable platelet-rich fibrin (i-PRF) is rich in growth factors (GFs) has been used for the repair regeneration of diabetic wounds; however, direct application i-PRF certain disadvantages, including instability bioactive molecules. Sericin hy...

OBJECTIVE The purpose of this study was to enhance the findings of an earlier ultrasound-induced lung hemorrhage study (Ultrasound Med Biol 2003; 29:1625-1634) that estimated pressure thresholds as a function of pulse duration (PD: 1.3, 4.4, 8.2, and 11.6 micros; 2.8 MHz; 10-s exposure duration [ED]; 1-kHz pulse repetition frequency [PRF]). In this study, the roles of PRF and PD were evaluated ...

BACKGROUND Radiofrequency (RF) treatment is a minimally invasive procedure that has been used for more than 3 decades in treating various chronic pain syndromes. Conventional (continuous) RF treatment occasionally results in worsening or even initiating a new type of pain. The use of pulsed radiofrequency (PRF), which has a non- or minimally neurodestructive neuromodulatory effect, serves as an...