The protease of human immunodeficiency virus has been expressed in Escherichia coli and purified to apparent homogeneity. Immunoreactivity toward anti-protease peptide sera copurified with an activity that cleaved the structural polyprotein gag p55 and the peptide corresponding to the sequence gag 128-135. The enzyme expressed as a nonfusion protein exhibits proteolytic activity with a pH optim...

Protease activity has been demonstrated in culture supernatants of Clostridium tetani at various stages of fermentation. Gel chromatography of the concentrated filtrates revealed the presence of three enzymatically active fractions eluting at separate positions off the column. The smallest protease was found to “nick” the single chain intracellular tetanus toxin, producing the extracellular, tw...

A protease which was activated by SDS was purified to homogeneity from maize leaves. On the basis of its proteolytic activity towards ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco) or a synthesized peptide, the purification was carried out using immunoaffinity chromatography with a monoclonal antibody raised against a partially purified enzyme by native gradient PAGE. The purified p...

This study was conduction to find a suitable and cheap plant as a source for extracting vegetable protease enzymes and investigate the effect of these enzymes for producing whey protein hydrolysate. The extract prepared from the composite weed cichorium pumilum had a satisfactory protease activity, each ml of this extract prepared from 0.1 gm dried cichorium weed stems.An alkaline and acidic ci...

Protease enzymes are that able to hydrolyze peptide bonds in proteins into simple compounds. The protease enzyme used was derived from fermented sea cucumber digestive organs (HSFI-8). HSFI-8 uses meat tenderizer as well an anticoagulant agent. samples were beef, chicken and tuna whose protein profile analyzed using SDS-PAGE. Three types of soaked at a concentration 30% v/v for 3 h. In addition...

OBJECTIVES Lipolytic genes have been investigated in several viral genomes, and some of them show enzyme activity which can be used for various functions including the production of DNA replication metabolites, rescue from endosomes, and membrane fusion. Amsacta moorei entomopoxvirus (AMEV) replicates in nearly the entire insect body, especially in the adipose tissue. One of the open reading fr...

Cow dung, a cheap and easily available source of energy, was used as the substrate for the production of alkaline protease by solid-state fermentation using the Bacillus subtilis strain VV. In order to achieve the maximum yield of this enzyme, the following optimum process parameters are needed: fermentation period (72 h), pH (10.0), moisture content (140%), inoculum (25%), temperature (30-40°C...

A new protease which specifically acts on the apo-form of pyridoxal enzymes was discovered in rat small intestine and skeletal muscle. This enzyme was puriCed about SM-fold from the small intestine of vitamin Be-deficient rats. Some proporties of the enzyme and its action were studied. The enzyme split the apo-protein of ornithine transaminase into two products, a homogeneous smaller protein an...

An alkali tolerant Bacillus strain having extracellular serine alkaline protease activity was newly isolated from compost and identified as Bacillus clausii GMBE 22. An alkaline protease (AP22) was 4.66-fold purified in 51.5% yield from Bacillus clausii GMBE 22 by ethanol precipitation and DEAE-cellulose anion exchange chromatography. The purified enzyme was identified as serine protease by LC-...

Alkaline protease enzyme was produced under solid state fermentation by Fusarium solani. Various agro-wastes were evaluated to check their potential utilization in the solid state fermentation for protease production. In this connection, the results showed that rice straw was the best agro-waste. The best enzyme activity was achieved at 7th day of incubation, pH 8, temperature 45 °C, inoculum’s...