The skeletal isoenzyme of human alkaline phosphatase was partially purified from the serum of a patient with Paget’s disease. The pH dependence of the reaction with p-nitrophenyl phosphate (PNPP) suggests that either the reaction mechanism involves 2 dissociable residues on the enzyme (8.75 > pK1 > 8.2; 9.35 > pKz > 8.75), or that the monoanion is the active form of the phosphoryl substrate. In...

As an important industrial enzyme, protease is widely used in feed, food and other fields. At present, the insufficient activity obtained from microorganisms cannot meet purpose of production. In this study, Bacillus amyloliquefaciens with high production was screened animal feces by plate transparent circle method. To improve protease, atmospheric room temperature plasma (ARTP) mutagenesis fir...

background: proteolytic enzymes have an important role in variety of physiological and pathological functions. they have been used in therapeutic and pharmaceutical applications. characterizations of extracellular proteases from various strains of s. marcescens indicate that most strains produce a very similar major metalloprotease. this metalloprotease (serrapeptidase, serrapeptase) is an impo...