For many protein families, such as serine proteinases or serine proteinase inhibitors, the family assignment predicts reactivity only in general terms. Both detailed specificity and quantitative reactivity are lacking. We believe that, for many such protein families, algorithms can be devised by defining the subset of n functionally important sequence positions, making the 19n possible single m...

When a tomato (Lycopersicon esculentum) leaf cDNA library was screened with a cDNA fragment from a polymerase chain reaction prepared using a degenerate primer mixture that was based on the amino acid sequence of tomato systemin (Pearce et al., 1991), a clone (LCYP-2) was obtained of 1295 bp, not including the 3’-terminal poly(A+) tail (Table I). It is surprising that when sequenced the clone a...

T h e commonly used trypsin substrate Na-benzoyl-m.arginine p-naphthylamide ( B A N A ) was not cleaved by either GB or SCTPA. BANA caused 50% inhibition of G B at 2 x 1 0 F J M when assayed with MUGB, and 50% inhibition of SCTPA at lo-’ M when assayed with substrate S-2288. We conclude that the proteinases SCTPA and GB (although not necessarily identical) are more closely related t o each othe...