Box H/ACA ribonucleoprotein particles (RNPs) mediate pseudouridine synthesis, ribosome formation, and telomere maintenance. The structure of eukaryotic H/ACA RNPs remains poorly understood. We reconstituted functional Saccharomyces cerevisiae H/ACA RNPs with recombinant proteins Cbf5, Nop10, Gar1, and Nhp2 and a two-hairpin H/ACA RNA; determined the crystal structure of a Cbf5, Nop10, and Gar1 ...

A multifunctional surface that enables control of wetting, optical reflectivity and mechanical damage of nanostructured interfaces is presented. Our approach is based on imprinting a periodic array of nanosized cones into a UV-curable polyurethane acrylate (PUA), resulting in a self-reinforcing egg-crate topography evenly distributed over large areas up to several cm(2) in size. The resulting s...

The structure of dual ascent methods is particularly well-suited for taking advantage of good initial dual solutions of minimum cost flow problems. For this reason, these methods are extremely efficient for reoptimization and sensitivity analysis. In the absence of prior knowledge of a good initial dual solution, one may attempt to find such a solution by means of a heuristic initialization. RE...

AIMS To compare amount of root canal debris and irrigant extruded apically after irrigants agitation using closed and open chambers. METHODS AND MATERIAL Sixty maxillary central incisors were selected, decoronated, and mounted in preweighed glass vials filled with distilled water. Biomechanical preparation was completed using ProTaper rotary files until number F4 and 1 mL of 3% NaOCl solution...

For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xylose-utilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and...

Mechanism of Its Antilipolytic Action 1 T. R. TEPHLY AND G. J. MANNERING. Inhibition of Drug Metabolism. V. Inhibition of Drug Metabolism by Steroids 10 R. E. STITZEL, T. R. TEPHLY, AND G. J. MANNERING. Inhibition of Drug Metabolism. VI. Inhibition of Hexobarbital Metabolism in the Isolated Perfused Liver of the Rat 15 G. ETZOLD, B. PREUSSEL, AND P. LANGEN. Structural Requirements for the Inhib...

The methylation of U1498 located in the 16S ribosomal RNA of Escherichia coli is an important modification affecting ribosomal activity. RsmE methyltransferases methylate specifically this position in a mechanism that requires an S-adenosyl-L-methionine (AdoMet) molecule as cofactor. Here we report the structure of Apo and AdoMet-bound Lpg2936 from Legionella pneumophila at 1.5 and 2.3 Å, respe...