In 1942 pyridoxine was related to protein metabolism (1). Since that time this vitamin or a derivative thereof has been described as a component of transaminase (2) and of decarboxylase (3). Pyridoxine has been claimed to be beneficial in the treatment of a variety of diseases. An attempt to produce a deficiency of this vitamin in a human subject failed to give evidence of any significant chang...

In 1942 pyridoxine was related to protein metabolism (1). Since that time this vitamin or a derivative thereof has been described as a component of transaminase (2) and of decarboxylase (3). Pyridoxine has been claimed to be beneficial in the treatment of a variety of diseases. An attempt to produce a deficiency of this vitamin in a human subject failed to give evidence of any significant chang...

y-Aminobutyric acid is present in the free form in large amounts in brain (l-3), where it is formed from glutamic acid by a L-glutamic acid decarboxylase (2, 4, 5). The enzyme requires pyridoxal phosphate as coenzyme (5), which can be synthesized in brain from adenosinetriphosphate and pyridoxal (6). This decarboxylase has a high degree of substrate and coenzyme specificity (6). The concentrati...

CONTEXT AND OBJECTIVE The coenzyme of aspartate aminotransferase is pyridoxal phosphate, generated from fresh vegetables containing pyridoxine. Vitamin B6-responsive sideroblastic anemia, myelofibrosis and Peyronies syndrome respond to high pyridoxine doses. The objective was to investigate the oral pyridoxine oral dose that would lead to maximized pyridoxal phosphate saturation of red cell asp...

In uitro, isolated choroid plexuses, the anatomical location of the system that transports vitamin I~,$ (BJ from blood into cerebrospinal fluid, and brain slices concentrated :‘H from media containing [3H]pyridoxine. Within these tissues, most of the intracellular [:‘H]pyridoxine was converted to and retained as the phosphorylated [“HIB,; vitamers. However, isolated choroid plexuses and brain s...

The kidneys play an important role in regulating vitamin B(6) body homeostasis, but limited information exists regarding the mechanism of pyridoxine uptake by renal epithelial cells, and no study exists on its regulation. To address these issues, we used the renal opossum-derived tubular epithelial (opossum kidney; OK) cells and found pyridoxine uptake to 1) be temperature and energy dependent,...

Amiloride, a diuretic drug that acts by inhibition of various sodium transporters, is toxic to the fission yeast Schizosaccharomyces pombe. Previous work has established that amiloride sensitivity is caused by expression of car1+, which encodes a protein with similarity to plasma membrane drug/proton antiporters from the multidrug resistance family. Here we isolated car1+ by complementation of ...

The Cercospora nicotianae SOR1 (singlet oxygen resistance) gene was identified previously as a gene involved in resistance of this fungus to singlet-oxygen-generating phototoxins. Although homologues to SOR1 occur in organisms in four kingdoms and encode one of the most highly conserved proteins yet identified, the precise function of this protein has, until now, remained unknown. We show that ...

Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile ph...