Carbox dioxide fixation studies were conducted with rapidly growing JA-1 sarcoma cells in vitro. Isotope was isolated in asparagine, aspartic acid, glutamic acid, and glutamine. Chemical degradation of these compounds suggested three sites of fixation. They were carbon dioxide condensation with (a) pyruvic acid, (6) propionyl CoA, and (c) a three-carbon fragment such as the isopropyl carbons of...

Almost all enzymes capable of decarboxylating pyruvate have been shown to catalyze DPT’-dependent condensation reactions yielding acyloins such as AMC (l-7). Recent work of Schweet and coworkers (8) has demonstrated that in animal tissues these condensations are due to the activity of pyruvic oxidase. Purified preparations of pyruvic oxidase from pigeon breast muscle have the same ratio of acyl...

Polycyclic Aromatic Hydrocarbons (PAHs) are complex and widely distributed environmental pollutants that can affect living ecosystems. This study was conducted to rapidly degrade phenanthrene and pyrene representing low and high molecular weight of PAHs, respectively. Cronobacter sakazakii MM045 (KT933253) was identified from used engine oil of contaminated soil. PAHs biodegradation was carried...