Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different steps in the qRT-PCR reaction have been reported; however, statistical analysis and presentation of substantially variable biological repeats present problems and are often not meaningful, for example...

Purpose: To investigate the effect of tripartite motif-containing protein 8 (TRIM8) on contrast media-induced injury kidneys in mice and human renal tubular epithelial cells.
 Methods: Contrast nephropathy (CIN) model was constructed by injecting meglumine diatrizoate mice. Small interference technology used to knock down expression TRIM8 HK-2 cells, while quantitative real-time polymerase...