Using affinity purified human immunodeficiency virus (HIV) reverse transcriptase the reaction assay conditions were determined. The optimum incorporation of dTMP into the (rA)n(dT)10 template with HIV reverse transcriptase required 6 mM MgCl2 and 80 mM KCl. The template specificity of HIV reverse transcriptase is quite different from those of the human gamma-polymerase-associated reverse transc...

with development of technology and science and continuous increase in productivity, product life cycles are shortened and customer demands are more personalized and diversified, which makes companies face with ever changing and unpredictable competitive environment. therefore, rapid response to market competition has become one of the main focuses and the competitive advantages. today, one of t...

Liminality refers to being “betwixt and between” (Turner, 1967). We draw on boundary theory propose elaborate upon the notion of “liminal researchers”—the in-between status we hold when conduct research that is personally meaningful or in highly familiar settings, despite fact are not actually a member group studied. Indeed, liminal researcher organizational studies denotes dwelling phenomenon ...

Let R be a ring and consider the category ModR of (right) R-modules. Given a class C ofR-modules, a morphismM → N in ModR is called a left C-approximation of M provided that N belongs to C and the induced morphism HomR(N,C) → HomR(M,C) is surjective for every C in C. This concept has been introduced by Auslander and Smalø [2], and independently by Enochs [5]. Approximations arise naturally in v...