We have investigated the role of Caenorhabditis elegans RAD-51 during meiotic prophase and embryogenesis, making use of the silencing effect of RNA interference (RNAi). rad-51 RNAi leads to severe defects in chromosome morphology in diakinesis oocytes. We have explored the effect of rad-51 RNAi in mutants lacking fundamental components of the recombination machinery. If double-strand breaks are...

This paper describes the introduction of synchrotron-based macromolecular crystallography (MX) into an undergraduate laboratory class. An introductory 2 week experimental module on MX, consisting of four laboratory sessions and two classroom lectures, was incorporated into a senior-level biochemistry class focused on a survey of biochemical techniques, including the experimental characterizatio...

Let G be a finite group and k a fixed algebraically closed field of characteristic p>0 . If p is odd, let H(; be the subring of H*(G,k) consisting of elements of even degree; following [20-22] we take H~=H*(G,k) if p=2, though one could just as well use the subring of elements of even degree for all p. H a is a finitely generated commutative k-algebra [13], and we let Va denote its associated a...

Abstract. In this article, we study tensor product of Hilbert C∗-modules and Hilbert spaces. We show that if E is a Hilbert A-module and F is a Hilbert B-module, then tensor product of frames (orthonormal bases) for E and F produce frames (orthonormal bases) for Hilbert A⊗B-module E ⊗F , and we get more results. For Hilbert spaces H and K, we study tensor product of frames of subspaces for H an...

BACKGROUND Rad (Ras associated with diabetes) GTPase is the prototypic member of a subfamily of Ras-related small G proteins. The aim of the present study was to define whether Rad plays an important role in mediating cardiac hypertrophy. METHODS AND RESULTS We document for the first time that levels of Rad mRNA and protein were decreased significantly in human failing hearts (n=10) compared ...

BACKGROUND AND OBJECTIVES Modern imaging techniques have increased the incidental detection of renal atherosclerotic disease (RAD). Because immune activation may hasten RAD progression, identifying cellular immune markers might provide clues to clinical activity. In this study, cellular immune markers were assessed in early RAD. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS Immune cell marker...

BACKGROUND The small GTPase Rad is a negative regulator of voltage-dependent L-type calcium channel current (ICaL); however, the effects of Rad ablation on cardiomyocyte function are unknown. The objective of this study is to test the hypothesis that Rad-depletion causes positive inotropic effects without inducing cardiac hypertrophy. METHODS AND RESULTS Ventricular myocytes from adult Rad(-/...

A recombinant adenovirus (rAd) expressing Cre recombinase derived from bacteriophage P1 has already been extensively used for the conditional gene activation and inactivation strategies in mammalian systems. In this study, we generated AxCAFLP, a rAd expressing FLP recombinase derived from Saccharomyces cerevisiae and carried out quantitative comparisons with Cre-expressing rAd in both in vitro...

Disorders of L-type Ca channels can cause severe cardiac arrhythmias. A subclass of small GTP-binding proteins, the RGK family, regulates L-type Ca current (ICa,L) in heterologous expression systems. Among these proteins, Rad (Ras associated with diabetes) is highly expressed in the heart, although its role in the heart remains unknown. Here we show that overexpression of dominant negative muta...

g t l n T o put into deserved perspective the contribution that Gleason and colleagues make in this issue of the Journal, theirs is one of the most extensive validation studies to date of reactive attachment disorder (RAD). The investigators sought to validate RAD with approaches derived from the classic 1970 article by Robins and Guze. The study focused primarily on four complementary aspects ...