In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA  sequencing, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of Ind...

DOI: 10.1002/sscp.202200019 The cover picture shows that the lead ion (Pb2+) and copper (Cu2+) in lake water are detected by UV-Vis analysis using gold nanoparticles (Au NPs) as colorimetric probe. formation of Pb-O bond between Pb2+ L-Cysteine can induce significant aggregation Au NPs (blue color), conversely, Cu catalyze oxidation Cysteine prevent (pink color). By analyzing absorption spectra...

Single-strand conformation polymorphism (SSCP) analysis is a simple and quick method for mutation detection. It depends on the separation of single-stranded (ss)DNA fragments in non-denaturing polyacrylamide gels. On occasion, the ssDNA bands can be very diffuse or appear against a heavily smeared background. Here, we demonstrate that the addition of denaturants, urea or formamide, to the gels ...

the drb3 gene is a highly polymorphic major histocompatibility complex (mhc) class ii gene and plays an important role in variability of immune responsiveness and disease resistance. in the present study, the mhc class ii drb3 gene in water buffalo (bubalus bubalis) populations from northwest regions of iran was investigated through pcr-sscp. genomic dna was extracted from whole blood samples c...

Familial hypercholesterolaemia (FH) is an autosomal codominant disorder characterised by high levels of LDL cholesterol and a high incidence of coronary artery disease. Our aims were to track the low density lipoprotein receptor (LDLR) gene in individual families with phenotypic FH and to identify and characterise any mutations of the LDLR gene that may be common in the west of Scotland FH popu...

PURPOSE To assess the allelic variation of the VMD2 gene in patients with Best disease and age-related macular degeneration (AMD). METHODS Three hundred twenty-one AMD patients, 192 ethnically similar control subjects, 39 unrelated probands with familial Best disease, and 57 unrelated probands with the ophthalmoscopic findings of Best disease but no family history were screened for sequence v...

The reference standard methods for drug susceptibility testing of Mycobacterium tuberculosis, such as culture on Lowenstein-Jensen or Middlebrook 7H10/11 medium, are very slow to give results; and due to the emergence of multidrug-resistant M. tuberculosis and extensively drug-resistant M. tuberculosis, there is an urgent demand for new, rapid, and accurate drug susceptibility testing methods. ...

To explore whether the crenarchaeal consortium found in the rhizosphere is distinct from the assemblage of crenarchaeotes inhabiting bulk soil, PCR-single-stranded-conformation polymorphism (PCR-SSCP) profiles were generated for 76 plant samples collected from native environments. Divergent terrestrial plant groups including bryophytes (mosses), lycopods (club mosses), pteridophytes (ferns), gy...

Lactoferrin is an iron binding glycoprotein which plays an essential role in antimicrobial defense and a potential candidate gene in dairy cattle breeding. The present study was undertaken with the objective of sequence characterization and identification of polymorphisms within exons 2, 3 and 14 and their flanking intronic regions in lactoferrin gene of Deoni (Bos indicus) cattle by single str...

Tuberous sclerosis is an autosomal dominant multisystem disorder characterized by hamartomatous growths in different organs. Disease determining genes are localized to 9q34 (TSC1) and 16p13.3 (TSC2). Two-thirds of the cases are sporadic and result from new mutations. The aim of this study was to determine TSC2 gene mutations by Single Stranded Conformation Polymorphism (SSCP) analysis and direc...