Cenchrus is an important component of major grass cover of world. Similar to the other major tropical grasses most of the species in genus Cenchrus are also apomictic in nature hence correct and precise identification of accessions and species are problematic and dubious. In the present study 187 decamer oligonucleotide primers were tested for PCR-based DNA amplification of six prominent specie...

Phenotypic and genotypic characteristics of Salmonella Typhi were studied in 30 strains, isolated in different years, from some areas in Brazil. Conventional typing methods were performed by biochemical tests, Vi phage-typing scheme, and antimicrobial susceptibility test. Molecular typing methods were performed by analysis of plasmid DNA and by random amplified polymorphic DNA (RAPD-PCR). For t...

Two Saccharomyces cerevisiae strains were tested as the starter yeasts in a traditional cachaça distillery. The strains used were S. cerevisiae UFMG-A829, isolated from a cachaça fermentation process, and S. cerevisiae K1V1116, obtained from the wine industry. The permanence of each strain in the fermentation must was determined by RAPD (Random Amplified Polymorphic DNA)-PCR, with primer M13. B...

Two atypical Paracoccidioides brasiliensis strains (yeast form at room temperature) have been isolated from chronically infected patients living in Brazil. Different random primers were used to characterize these isolates and compare them to typical strains. The RAPD patterns allowed the differentiation of all the selected isolates. Their genetic distance ranged from 5% to 80% of non-shared ban...

ABSTRACT Visible and nonvisible quiescent infections of immature and mature fruit are an integral component of the disease cycle of brown rot of sweet cherry in California. Detection of these infections is critical for developing efficient and efficacious fungicide management programs. The previously published DNA amplification primers mfs3 and NS5 for the identification of Monilinia fructicola...

saffron (crocus sativus) is the most valuable and indigenous crop in iran. the stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. however, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. identification of these frauds with conventional and biochemical methods is difficult and low sensitive. therefore, ...

RAPD-PCR genetic markers were used to assess variation in wheat plants and connections among six genotypes. Four random primers produced 140 DNA fragments, averaging 6.7 identifiable bands per primer. Among the genotypes, 85 pieces (44.64 percent) polymorphic. Several RAPD marker had distinct signify recurrence patterns that thing differently amongst germplasm of groupings. Within-community acc...

Propagation through tissue culture by using orchid seed as explants will produce a lot of plants. This study aims to measure the genetic character plantlets that were regenerated from seeds which have been resulted in vitro culture. The original plants produced was determined Random Amplified Polymorphic DNA (RAPD) molecular markers. results showed primers used RAPD analysis polymorphic band pa...

background and objectives: urinary tract infection (uti) is one of the most common infections and a major cause of patient morbidity world wide. the gold standard for detection of bacterial pathogens in uti is culture of urine specimens. in order to facilitate identification of bacterial pathogens in clinical urine specimens we designed a molecular method to detect bacterial pathogen in uti. ma...

Backgrounds and Aims: Coxsakievirus B3 (CVB3), one of the six Coxsakievirus B serotypes, is a member of the Enterovirus genus within the Picornaviridae family. CVB3 is an important pathogen of viral myocarditis, which accounts for more than 50% of viral myocarditis cases. The genome of CVB3, like that of other Entroviruses, is a single-stranded, sense, polyadenylated RNA molecule with 7400 nucl...