color from ammonia was produced by an additional 5 min of incubation at 37 C. All strains of Proteus, except those of P. inconstans, generated detectable amounts of ammonia (Table 1). None of the Escherichia organisms was urease-positive. These findings were identical with those yielded by the urease technique of Stuart et al. The sensitivity of the Berthelot color reaction permits detection of...

helicobacter pylori infection is one of the most common human infections which have been associated with many upper gasterointestinal complications. different treatment regimens for helicobacter pylori eradication have been used. one of these regimes is the quadruple therapy regimen which metronidazole is one of the medications. in recent years several reports on h. pylori resistance to this an...

A simple, modified formation for urea broth gave consistent, reliable results to aid in the differentiation of Mycobacterium species. In a study of 1,346 isolates representing 17 different species, tests read after 7 days were distinct and reproducible. The use of this test facilitates the identification of Mycobacterium species, such as M. scrofulaceum, the M. avium complex, the M. terrae comp...

An evaluation of a rapid test for the presence of Helicobacter pylori from stool specimens looking for antigen proceeded for a period of 8 months from March to November 2011. A total of 212 stool samples were collected from 2 different hospitals in Holy Makkah City. Twenty six patients were positive for H. pylori by the ELISA test which was used as the gold standard. The rapid test when compare...

BACKGROUND Helicobacter pylori infection is etiologically associated with some important health problems such as gastric cancer. Because of the high clinical importance of H. pylori infection, development of a noninvasive test for the detection of H. pylori is desirable. METHODS In this study, a loop-mediated isothermal amplification (LAMP) targeted ureC of H. pylori was evaluated on 100 stoo...

BACKGROUND Helicobacter pylori is implicated as the causative agent for most duodenal and gastric ulcers. Invasive (endoscopy and biopsy) and noninvasive (serology, breath test) methods are currently available for definitive diagnosis of infectious peptic ulcer disease. METHODS Twenty-six patients with chronic gastritis symptoms underwent upper endoscopy, biopsy, rapid urease test, and [14C]u...

Helicobacter pylori survives and grows at low pHs via acid acclimation mechanisms that enable periplasmic pH homeostasis. Important components include a cytoplasmic urease; a pH-gated urea channel, UreI; and periplasmic α-carbonic anhydrase. To allow the rapid adjustment of periplasmic pH, acid acclimation components are recruited to the inner membrane in acid. The ArsRS two-component system, i...

PURPOSE We investigated the positivity rate and the time period to the positive color change of the urease test in children and adults and assessed the correlation of the urease test to histopathologic findings. METHODS From 1995 to 2000, endoscopic biopsies of the antrum and body were collected from 811 children and 224 adults and subjected to urease tests and histopathology. RESULTS The p...