BACKGROUND Our previous study showed that kallikrein gene transfer protects against gentamicin-induced nephrotoxicity and enhances renal function. In this study, we investigated the effects and potential mechanisms of kallikrein/kinin on inflammation and apoptosis induced by gentamicin. METHODS Rats were injected subcutaneously with gentamicin daily for 10 days and received an intravenous inj...

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

application of therapeutic gene transfer in the treatment of genetic diseases is a notable progress but there are some disadvantages and limitations in it. the process of overcoming these barriers is a drastic change in gene delivery. recently, calcium phosphate nanoparticles alone, or in combination with viral and nonviral vectors, were found to have a positive effect on gene transfer especial...

introduction: estradiol is a neuroactive steroid, which is found in several brain areas such as locus coeruleus (lc). estradiol modulates nociception by binding to its receptors and also by allosteric interaction with other membranebound receptors like glutamate and gabaa receptors. lc is involved in noradrenergic descending pain modulation. methods: in order to study the effect of 17β-estradio...

RESEARCH DESIGN AND METHODS—Reversa mice are LDL receptor–deficient mice that develop atherosclerosis. Their elevated plasma LDL levels are lowered after conditional knockout of the gene encoding microsomal triglyceride transfer protein. We examined the morphologic and molecular changes in atherosclerotic plaques in control and streptozotocin-diabetic Reversa mice after LDL lowering. Bone marro...