So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic cleavage of gag is strongly antigenic and is as conserved and immunogenic as p24. In some cases, antibodies to p17 are more prevalent than antibodies to p24 and the decline in...

The term biobetter also known as biosuperior which refers to a recombinant protein drug that is in the same class as an existing biopharmaceutical but is not identical; it is improved over the original. Biobetters are better in one or more product characteristics. Product individuality often targeted by biobetter applicants includes longer product half-life in the body, lower likelihood of aggr...

M.F. Desimone a,1, M.C. De Marzi b,1, G.J. Copello a, M.M. Fernández b, F.L. Pieckenstain c, E.L. Malchiodi b, L.E. Diaz a,∗ a Cátedra de Quı́mica Analı́tica Instrumental, Facultad de Farmacia y Bioquı́mica, Universidad de Buenos Aires, Junı́n 956 Piso 3◦ (1113) Ciudad de Buenos Aires, Argentina b Cátedra de Inmunologı́a, IDEHU-CONICET, Facultad de Farmacia y Bioquı́mica, Universidad de Buenos Aires,...

MATERIALS Taq DNA polymerase Marker DNA As a reference, use single-stranded DNA from the original bacteriophage M13 recombinant. MgCl2 (25 mM) Oligonucleotide primers The oligonucleotides should be 20-24 nucleotides in length, specific for the target DNA sequences, free of potential secondary structures, and contain no less than 10 and no more than 15 G and C residues. Yeast colony PCR buffer, ...

Address: 1Plant Cell Biology Laboratory, Instituto de Tecnologia Quimica e Biologica – UNL. Av. Republica, Apartado 127, 2781-901 Oeiras, Portugal, 2Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Quimica e Biologica – UNL. Av. Republica, Apartado 127, 2781-901 Oeiras, Portugal and 3Department of Plant Biology, Centro de Investigaciones Biológicas, CSIC. Ramiro de Maeztu 9, 28040 M...