Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

Background: The expression of bio-therapeutic proteins in mammalian cells, such as CHO, attains high homogeneity related to post-translational modifications. Although CHO remains the most popular cell line for bestselling biotherapeutic proteins on the market, there are still drawbacks such as expensive culture media, long time line, and high drug cost. Recently, researches on a novel Leishmani...

Background: DNA immunization is an appropriate method to produce an immunological response. Pseudomonas aeruginosa produces exotoxin A which is highly cytotoxic for eukaryotic cells. Since domains II (translocation domain) and 1b of the toxin have antigenic qualities, so they could be  useful candidates to protect against pseudomonas infections. Objectives: To evaluate if recombinant plasmid co...

Background: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB). Bacille Calmette-Guerin (BCG) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult TB vaccine. The aim of this study was to develop a new tuberculosis DNA vaccine candidate encoding a recombinant HspX-PPE44-EsxV fusion antigen of M. tuberculosis. Methods: ...

Background: Tuberculosis as a global health problem requires special attention in the contexts of prevention and control. Subunit vaccines are promising strategies to protect burdens of tuberculosis via increasing the BCG protection. The present study aimed to design a vaccine study by means of high-throughput expression and correct refolding of recombinant protein PPE17. Methods: We aimed to c...

objectives: in present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. materials and methods: we have cloned bovine prochymosin gene in methylotrophic yeast, p. pastoris, using ppic9k as an expression vector. the recombinant plasmid was transformed into the host by electroporation, and it was expressed in optimum con­ditions...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

Here we describe a half-semester biochemistry laboratory course based on the expression, isolation, purification, and biochemical characterization of RecJ nuclease. The students transformed expression plasmid thermostable nuclease into host bacteria Escherichia coli BL21(DE3), to induce recombinant by IPTG. expressed was purified from cell lysates broken with sonication Ni-affinity chromatograp...