background: spinach is one of the vegetables with a high concentration of nitrate which is affected by various processes such as cooking and refrigeration before consumption. the present study aimed to investigate the effect of home cooking method, which has not been studied before, on the level of nitrate and nitrite in spinach. the effect of refrigeration was studied, as well.   methods: afte...

Reduction of nitrate has been employed by several workers as a test for distinguishing species in the genus Brucella (cf Huddleson, 1943). We examined this test briefly during our studies on the dye sensitivity of brucellae (Pickett et al., 1952) and concluded that it should serve at least as a useful guide for identification of the species. However, further study disclosed that the outcome of ...

The unicellular green alga, Monoraphidium braunii, reduced extracellular ferricyanide at high rates. Impermeability of the membranes to ferriand ferrocyanide was shown for untreated cells as for those permeabilized with 5% I-propanol. Fe starvation stimulated reduction rates by 50%, light or glucose were ineffective. Extracellular acidification largely corresponded to formation of the new anion...

The aim of this study was to investigate nitrification activity of urea and the assimilation of nitrate in a well aerated soil using perfusion technique with addition of glucose as an energy and carbon source. In this study, urea was rapidly nitrified by the bacteria in the saturated soil but its course of transformation to NO3 was not linear. There was an initial increase in the concentration ...

The denitrifying bacterium Paracoccus denitrificans can grow aerobically or anaerobically using nitrate or nitrite as the sole nitrogen source. The biochemical pathway responsible is expressed from a gene cluster comprising a nitrate/nitrite transporter (NasA), nitrite transporter (NasH), nitrite reductase (NasB), ferredoxin (NasG) and nitrate reductase (NasC). NasB and NasG are essential for g...

Under anoxic conditions, zerovalent iron (Fe(0)) reduces nitrate to ammonium and magnetite (Fe3O4) is produced at near-neutral pH. Nitrate removal was most rapid at low pH (2-4); however, the formation of a black oxide film at pH 5 to 8 temporarily halted or slowed the reaction unless the system was augmented with Fe(2+), Cu(2+), or Al(3+). Bathing the corroding Fe(0) in a Fe(2+) solution great...

Methylophaga nitratireducenticrescens JAM1 is the only reported Methylophaga species capable of growing under anaerobic conditions with nitrate as electron acceptor. Its genome encodes a truncated denitrification pathway, which includes two nitrate reductases, Nar1 and Nar2; two nitric oxide reductases, Nor1 and Nor2; and one nitrous oxide reductase, Nos; but no nitrite reductase (NirK or NirS)...

Induction, energy gain, effect on growth, and interaction of nitrate and nitrite reduction of Bradyrhizobium sp. (Lupinus) USDA 3045 were characterized. Both nitrate and nitrite were reduced in air, although nitrite reduction was insensitive to ammonium inhibition. Anaerobic reduction of both ions was shown to be linked with energy conservation. A dissimilatory ammonification process was detect...

purpose: to investigate aqueous humor nitric oxide (no) levels in patients with branch retinal vein occlusion (brvo) and central retinal vein occlusion (crvo) and to compare these with age-matched controls. methods: eight consecutive patients with brvo and 16 patients with crvo were included in this study. aqueous humor specimens were obtained within 21 days of diagnosis. samples of aqueous hum...

An indirect method for monitoring nitric oxide (NO) by determining nitrate and nitrite using microchip capillary electrophoresis (CE) with electrochemical (EC) detection has been developed. This method combines determination of nitrite by direct amperometric detection following a microchip-based CE separation and conversion of nitrate to nitrite by chemical reduction using Cu-coated Cd granules...