An important goal is to identify the direct activation domain (AD)-interacting components of the transcriptional machinery within the context of native complexes. Toward this end, we first demonstrate that the multisubunit TFIID, SAGA, mediator, and Swi/Snf coactivator complexes from transcriptionally competent whole-cell yeast extracts were all capable of specifically interacting with the prot...

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pat...

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to ...

Intracellular delivery of functional macromolecules using peptide transduction domains (PTDs) is an exciting technology with both experimental and therapeutic applications. Recent data indicate that PTD-mediated transduction occurs via fluid-phase macropinocytosis involving an intracellular pH drop to approximately 5. Nitrilotriacetic acid (NTA)-coordinated metals avidly bind hexahistidine-tagg...

This paper briefly presents the canonical HPSG treatment of predicate complements (Section 1) and points out a number of problems with it (Section 2). Then it presents an alternative (Section 3) and shows how it avoids or solves the problems with the canonical treatment (Section 4). 1 The raising treatment of the predicate complements In mainstream HPSG predicate complements are treated along t...

Were we required to characterise this age of ours by any single epithet, we should be tempted to call it, not an Heroical, Devotional, Philosophical, or Moral Age, but, above all others, the Mechanical Age. It is the Age of Machinery, in every outward and inward sense of that word; the age which, with its whole undivided might, forwards, teaches and practises the great art of adapting means to ...

Imagine a poll to choose the best-known mathematician of the twentieth century. No doubt the winner would be John von Neumann. Reasons are seen, for instance, in the title of the excellent biography [M] by Macrae: John von Neumann. The Scientific Genius who Pioneered the Modern Computer, Game Theory, Nuclear Deterrence, and Much More. Indeed, he was a fundamental figure not only in designing mo...

This article investigates V. P. Aksyonov’s argument with L. N. Tolstoy about the role of personality in history his trilogy Generations Winter. The saga protagonists’ spiritual awakening and self-identification are discussed context Tolstoy’s view on activity general mass people who take part a historical event determine its outcome. allows to elicit man’s personal responsibility under Stalinis...

In this demo, we present GENIUS, a tool that facilitates research in the area of bilateral multi-issue negotiation. It implements an open architecture allowing easy development and integration of existing negotiating agents. GENIUS can be used to simulate individual negotiation sessions as well as tournaments between negotiating agents in various negotiation scenarios. It allows the specificati...