نتایج جستجو برای: rep and box primers

تعداد نتایج: 16839713  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2005
Katherine M Brendza Wei Cheng Christopher J Fischer Marla A Chesnik Anita Niedziela-Majka Timothy M Lohman

DNA helicases catalyze separation of double-helical DNA into its complementary single strands, a process essential for DNA replication, recombination, and repair. The Escherichia coli Rep protein, a superfamily 1 DNA helicase, functions in DNA replication restart and is required for replication of several bacteriophages. Monomers of Rep do not display helicase activity in vitro; in fact, DNA un...

2012
Qun Chen Thomas Ross Ying Hu Edward J. Lesnefsky

Myocardial injury is increased in the aged heart following ischemia-reperfusion (ISC-REP) compared to adult hearts. Intervention at REP with ischemic postconditioning decreases injury in the adult heart by attenuating mitochondrial driven cell injury. Unfortunately, postconditioning is ineffective in aged hearts. Blockade of electron transport at the onset of REP with the reversible inhibitor a...

Journal: :Scientific American 1906

2013
K. Chinda P. Luangpaiboon

This paper presents the comparative study of coded data methods for finding the benefit of concealing the natural data which is the mercantile secret. Influential parameters of the number of replicates (rep), treatment effects (τ) and standard deviation (σ) against the efficiency of each transformation method are investigated. The experimental data are generated via computer simulations under t...

2005
Katherine M. Brendza Wei Cheng Christopher J. Fischer Marla A. Chesnik Anita Niedziela-Majka Timothy M. Lohman

2 DNA helicases catalyze separation of double helical DNA into its complementary single strands, a process essential for DNA replication, recombination, and repair. The E. coli Rep protein, a superfamily 1 (SF1) DNA helicase, functions in DNA replication restart and is required for replication of several bacteriophages. Monomers of Rep do not display helicase activity in vitro; in fact, DNA unw...

2016
Ariel Kushmaro Eitan Ben-Dov

Culture-independent methods, employed to study the diversity and complexity of microbial communities that are based on amplification of rRNA genes with universal primers, include gradient gel electrophoresis (denaturing or temperature), single-strand-conformation polymorphism, restriction fragment length polymorphism, qPCR and highthroughput DNA sequencing. Substituting one or more base(s) with...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2016
Minsik Kim Hee Jung Kim Sang Hyeon Son Hye Jin Yoon Youngbin Lim Jong Woo Lee Yeong-Jae Seok Kyeong Sik Jin Yeon Gyu Yu Seong Keun Kim Sangryeol Ryu Hyung Ho Lee

DNA-binding repressors are involved in transcriptional repression in many organisms. Disabling a repressor is a crucial step in activating expression of desired genes. Thus, several mechanisms have been identified for the removal of a stably bound repressor (Rep) from the operator. Here, we describe an uncharacterized mechanism of noncanonical DNA binding and induction by a Rep from the tempera...

2012
Hari S. Karki Bishnu K. Shrestha Jae Woo Han Donald E. Groth Inderjit K. Barphagha Milton C. Rush Rebecca A. Melanson Beom Seok Kim Jong Hyun Ham

Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly...

1998
David L. Detlefs K. Rustan M. Leino Greg Nelson

A central methodological problem in programming with multiple levels of abstractions is the loosely defined problem of rep exposure. This paper traces the problem of rep exposure to the precisely defined notion of abstract aliasing. The paper also outlines a statically-enforceable discipline for avoiding abstract aliasing, but the outline is incomplete.

1996
Robert Weida Eric Mays Robert Dionne Meir Laker Brian White Chihong Liang Frank J. Oles

1 Introduction K-Rep is an object-oriented knowledge representation system based on description logic Woods and Schmolze, 1992]. K-Rep provides a language to express descriptions (e.g., of medical concepts such as drugs, treatments, and diseases), inference mechanisms for reasoning about descriptions and their relationship to one another, and considerable supporting infrastructure. This paper p...

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