نتایج جستجو برای: rep and box primers
تعداد نتایج: 16839713 فیلتر نتایج به سال:
DNA helicases catalyze separation of double-helical DNA into its complementary single strands, a process essential for DNA replication, recombination, and repair. The Escherichia coli Rep protein, a superfamily 1 DNA helicase, functions in DNA replication restart and is required for replication of several bacteriophages. Monomers of Rep do not display helicase activity in vitro; in fact, DNA un...
Myocardial injury is increased in the aged heart following ischemia-reperfusion (ISC-REP) compared to adult hearts. Intervention at REP with ischemic postconditioning decreases injury in the adult heart by attenuating mitochondrial driven cell injury. Unfortunately, postconditioning is ineffective in aged hearts. Blockade of electron transport at the onset of REP with the reversible inhibitor a...
This paper presents the comparative study of coded data methods for finding the benefit of concealing the natural data which is the mercantile secret. Influential parameters of the number of replicates (rep), treatment effects (τ) and standard deviation (σ) against the efficiency of each transformation method are investigated. The experimental data are generated via computer simulations under t...
2 DNA helicases catalyze separation of double helical DNA into its complementary single strands, a process essential for DNA replication, recombination, and repair. The E. coli Rep protein, a superfamily 1 (SF1) DNA helicase, functions in DNA replication restart and is required for replication of several bacteriophages. Monomers of Rep do not display helicase activity in vitro; in fact, DNA unw...
Culture-independent methods, employed to study the diversity and complexity of microbial communities that are based on amplification of rRNA genes with universal primers, include gradient gel electrophoresis (denaturing or temperature), single-strand-conformation polymorphism, restriction fragment length polymorphism, qPCR and highthroughput DNA sequencing. Substituting one or more base(s) with...
DNA-binding repressors are involved in transcriptional repression in many organisms. Disabling a repressor is a crucial step in activating expression of desired genes. Thus, several mechanisms have been identified for the removal of a stably bound repressor (Rep) from the operator. Here, we describe an uncharacterized mechanism of noncanonical DNA binding and induction by a Rep from the tempera...
Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly...
A central methodological problem in programming with multiple levels of abstractions is the loosely defined problem of rep exposure. This paper traces the problem of rep exposure to the precisely defined notion of abstract aliasing. The paper also outlines a statically-enforceable discipline for avoiding abstract aliasing, but the outline is incomplete.
1 Introduction K-Rep is an object-oriented knowledge representation system based on description logic Woods and Schmolze, 1992]. K-Rep provides a language to express descriptions (e.g., of medical concepts such as drugs, treatments, and diseases), inference mechanisms for reasoning about descriptions and their relationship to one another, and considerable supporting infrastructure. This paper p...
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