Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

A Reverse Transcription-Polymerase Chain Reaction (RT-PCR) technique was applied for the detection of Potato leaf roll polerovirus (PLRV) in dormant potato tubers. A primer pair was designed from the coat protein-encoding fragment of the PLRV genome that amplified a 336-bp product. The amplified product was detected in nucleic acid preparations from leaves and tubers of 5 cultivars and from pur...

To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration method using sulfonated (SO-) magnetic beads in the presence of divalent cations. In the presence of either Zn(2+) or Cu(2+) ions, we showed that SO-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine par...

A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...

Despite routine vaccination programs against Newcastle disease (ND), sporadic cases have occasionally occurred that remain a constant threat to commercial poultry. Ten isolates of Newcastle disease viruses (NDV) from infected broiler chicken cases were obtained from various locations in Fars province during 2009-2011 and genetically analyzed using reverse transcription polymerase chain reaction...

We report a case of a 19-year-old woman with a primary pericardial synovial sarcoma that extended from the right ventricular free wall to the posterior aspect of the left anterior thoracic wall. Synovial sarcoma was diagnosed by the detection of the chimeric transcript SYT-SSX using reverse transcriptase-polymerase chain reaction (RT-PCR). This transcript is generated by reciprocal translocatio...

A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100% agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivit...

We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing unbound MB. This module consists microfluidic channel with shape funnel operated electrohydrodynamic actuation. report limit detection 2 pM in less than 1 min miR-21 alone, then demonstrate that levels, measured fine needle biopsy samples, fr...

Dengue fever is an emerging disease in Pakistan with uninterrupted increase in number of cases since 2006 Dengue fever epidemic in Lahore Pakistan. This study was planed to identify serotypes of dengue virus (DENV) involved in epidemic of 2008. The patients were screened in the microbiology laboratory with immunochromatographic kits then confirmed by antibody capture Enzyme linked immunosorbent...

In the present study a protocol of in situ reverse transcriptase-nested polymerase chain reaction (in situ RT-nested PCR) was examined based on the following modifications. (i) To exclude false positive signals caused by "DNA repair mechanisms" and "endogenous priming", a two-step PCR was applied after reverse transcription. The first step was performed in the presence of extrinsic primers and ...