Third, because DRB1 antigens are the most polymorphic and hyper variable of all the HLA antigens, reagents often give cross-reactions. As a result ofthese drawbacks, serological HLA-DRB1 typing is more demanding technically and gives up to 25% typing errors as compared with genotyping methods, making it less reliable (7, 8). Of the several available genotyping methods, DNA-RFLP analysis require...

A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different geographical origins (Japan, Italy, and Nepal) and hosts (humans, calves, and a goat). These isolates were characterized by means of DNA sequencing, PCR-restriction fragment length polymorphism (PCR-RFLP),...

Background: Type 2 diabetes mellitus that characterized by insulin resistance and it is a risk of many diseases the impact genetic factors on well documented. Vitamin D receptor (VDR) gene polymorphisms have been linked to T2DM. In this study, we analyzed relation between TaqI ApaI VDR T2DM subjects using PCR-RFLP method in Kurdish patients.

A new method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of a point mutation of the Bruton’s tyrosine kinase (Btk) gene in CBA/N mice bearing an X-linked recessive immunodeficiency (xid). Since restriction site useful for RFLP analysis does not exist in the spontaneous mutant Btk locus, an artificial restriction site...

INTRODUCTION The T to C transition at nucleotide 1565 of the human glycoprotein IIIa (ITGB3) gene represents a genetic polymorphism (PlA1/A2) that can influence both platelet activation and aggregation and that has been associated with many types of disease. Here, we present a newly designed multiplex tetra-primer amplification refractory mutation system - polymerase chain reaction (T-ARMS-PCR)...