The branched DNA (bDNA) assay is a reliable method for quantifying the RNA of human immunodeficiency virus type 1 (HIV-1). The positive controls and standards for this assay for the detection of HIV-1 consist of naked RNA, which is susceptible to degradation by RNase. Armored RNA is a good candidate for an RNase-resistant positive control or standard. However, its use has been limited by the ma...

Prunus fruit trees of the Rosaceae family exhibit S-RNase-based gametophytic self-incompatibility (GSI), which enables pistils to reject self-pollen by suppressing pollen tube elongation. In other plant species with GSI, it has been shown that this suppression consists two steps: first, slowing down elongation in middle part style and second, complete arrest involving programmed cell death. To ...

UNLABELLED THE BACKGROUND: Ribonuclease L (RNASEL), encoding the 2'-5'-oligoadenylate (2-5A)-dependent RNase L, is a key enzyme in the interferon induced antiviral and anti-proliferate pathway. Mutations in RNASEL segregate with the disease in prostate cancer families and specific genotypes are associated with an increased risk of prostate cancer. Infection by human papillomavirus (HPV) is the ...

RNase MRP is a site-specific ribonucleoprotein endoribonuclease that processes RNA from the mammalian mitochondrial displacement loop containing region. RNase P is a site-specific ribonucleoprotein endoribonuclease that processes pre-tRNAs to generate their mature 5'-ends. A similar structure for the RNase P and RNase MRP RNAs and a common cleavage mechanism for RNase MRP and RNase P enzymes ha...

The RNase activity and properties of ribosome and polysomc preparations from normal rat liver and some hepatomas havc been examined. Polysomc and ribosome preparations from the Novikoff, McCoy MDAB, and Dunning hcpatomas had considerably higher specific RNase activity than corresponding preparations from normal rat livcr, Novikoff ascites, or Morris 5123 hcpatomas. The optimum pH of the RNasc w...

The RNase activity and properties of ribosome and polysomc preparations from normal rat liver and some hepatomas havc been examined. Polysomc and ribosome preparations from the Novikoff, McCoy MDAB, and Dunning hcpatomas had considerably higher specific RNase activity than corresponding preparations from normal rat livcr, Novikoff ascites, or Morris 5123 hcpatomas. The optimum pH of the RNasc w...