نتایج جستجو برای: rolling circle amplification rca
تعداد نتایج: 101614 فیلتر نتایج به سال:
A new method to label and detect the long ssDNA products from rolling circle (RC) amplification was reported. ZnS nanocrystal clusters (NCCs) were used to tag the RC products. The NCCs release millions of cations to trigger millions of metal-responsive dyes for cascade signal amplification. Selective and sensitive quantification of miRNA was demonstrated; and the NCCs delivered much lower detec...
Lou et al. (1) report a technique for increased sensitivity of DNA sequencing that they call “circle sequencing.” The authors’ method involves circularizing single-stranded DNA and performing rolling-circle amplification (RCA). Multiple duplicates are thereby generated, and errors can be removed by comparing the sequences of the duplicates. Lou et al. report accuracy similar to that of singlest...
We have developed a rapid and high-throughput assay based on rolling circle amplification, to distinguish individual strand cleavage of DNA duplexes by restriction endonucleases. As an illustration, we analyzed nicking activity of Nb.BbvCI and uneven cleavage of LNA modified DNA by EcoRI. This assay has potential for analyzing protein-DNA interactions.
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