Affinity isolation has been an essential technique for molecular studies of cellular assemblies, such as the switch/sucrose non-fermentable (SWI/SNF) family of ATP-dependent chromatin remodeling complexes. However, even biochemically pure isolates can contain heterogeneous mixtures of complexes and their components. In particular, purification strategies that rely on affinity tags fused to only...

Typhoid fever is common in developing countries, with an estimated 120 million infections and 700 000 annual deaths, worldwide. Fluoroquinolones have been the treatment of choice for infection with multidrug-resistant (MDR) Salmonella enterica serovar Typhi (S. Typhi). However, alarming reports of fluoroquinolone-resistance and failure of typhoid fever treatment have recently been published. To...

OBJECTIVE To compare perioperative and postoperative surgical outcomes between and among open and minimally invasive sacrocolpopexies (MISCs). METHODS We performed a multicenter retrospective cohort study comparing abdominal sacrocolpopexy (ASC) and MISC from January 1999 to December 2010. RESULTS A total of 1124 subjects underwent sacrocolpopexy, with 589 ASCs and 535 MISCs. Within the MIS...

A membrane lipid abnormality induced by sickling and found as a permanent alteration in the irreversibly sickled cell (ISC) is the rearrangement of phosphatidyl ethanolamine (PE) and phosphatidyl serine (PS) from the inner to the exterior side of the lipid bilayer. Since PS can provide a catalytic surface for the binding of blood coagulation factors and thus can exhibit procoagulant activity, w...

Human imaging localizes most visceral nociceptive responses to anterior cingulate cortex (ACC), however, imaging in conscious subjects cannot completely control anticipatory and reflexive activity or resolve neuron activity. This study overcame these shortcomings by recording individual neuron responses in 12 anesthetized and paralyzed rabbits to define the visceronociceptive response pattern b...

Diante de um mercado cada vez mais exigente, consciente das necessidades e efeitos ações organizacionais, cresce o interesse evidenciação controle informações divulgadas. Assim, com objetivo investigar a influência da Responsabilidade Social Corporativa (RSC) na qualidade demonstrações contábeis empresas brasileiras, realizou-se uma pesquisa descritiva junto 300 listadas B3, aplicando-se estatí...

with the DNA. The stability of the nucleosome is reYahli Lorch, Mincheng Zhang, and Roger D. Kornberg* duced, but the gross structure of the nucleosome is Department of Structural Biology unaffected. This “activated” state of the nucleosome is Stanford School of Medicine dependent on the presence of the chromatin-remodelStanford, California 94305 ing complex but not on continuing ATP hydrolysis...

Alternative re-usable substrate containers for fructification are required because plastic bags currently used suffocate soil biotic entities. They are a cost for mushroom farmers who purchase plastic bags in every oyster mushroom crop and they are left as non-biodegradable wastes, which are disposed of to the environment after every oyster mushroom cropping. On the other hand, oyster mushroom ...

Many non-linear controllers have been used to improve system performances and robustness of double fed induction machine (DFIG) based wind turbines like sliding mode control (SMC). However, this type controller is less efficient due a sign function that can lead chattering phenomenon. To overcome deficiency, the replaced by fuzzy logic (FLC). This what proposed in paper which deals with doubly ...