Depletion of mitochondrial DNA (mtDNA) and mtDNA-encoded respiratory chain proteins in subcutaneous (SC) fat from patients with HIV lipoatrophy have clearly demonstrated the role of mitochondrial dysfunction in this syndrome. Research in HIV lipoatrophy, however, has been severely hampered by the lack of a suitable surrogate marker in blood or other easily obtained clinical specimens as fat bio...

Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. This is because the information contained within the genome is context-independent; i.e., generally, every normal cell contains the same DNA sequence--the same mutations and polymo...

Background and Aim: The purpose of this study was to investigate the formation of gap junctions and to characterize the expression of Connexin (CX) 43 during the proliferation of Malassez’s epithelial rest (MER) cells. Materials and Methods: MER cells derived from the porcine periodontal ligament were cultured in 35 mm dishes as 2D culture. MER cells mixed with type 1 collagen gels were also cu...

Existing estimates of the incidence of infectious intestinal disease (IID) caused by norovirus are based on electron microscopy or reverse transcription-polymerase chain reaction (RT-PCR). Neither method accurately represents norovirus disease burden: Electron microscopy has poor diagnostic sensitivity, and RT-PCR has poor diagnostic specificity. In this study, viral load measurements were used...

OBJECTIVE We investigated the capacity of first trimester peripheral blood mononuclear cell (PBMC) microRNA to determine risk of spontaneous preterm birth among pregnant women. STUDY DESIGN The study included 39 pregnant women with the following delivery outcomes: 25 with a full term delivery (38-42 weeks gestation) 14 with spontaneous preterm birth (<38 weeks gestation). Of the 14 women expe...

Although studies in vivo revealed promising results in bone regeneration after implantation of scaffolds together with osteogenic progenitor cells, basic questions remain how material surfaces control the biology of mesenchymal stem cells (MSC). We used human MSC derived from bone marrow and studied the osteogenic differentiation on calcium phosphate surfaces. In osteogenic differentiation medi...

Background: With the drastic spread of COVID-19 and mass mortality people globally, detection progression this disease has stood out to be a necessity. Hence, we set identify prevalence antibodies in Bangladesh using in-house rapid pan-immunoglobulin dot-blot test kit evaluate performance kit. Methods: In cross-sectional study, tested serum collected between mid-May mid-June 2020 for by RTPCR c...

Background: Since the COVID-19 pandemic began, medical students have been allotted various duties like rapid antigen testing, Sanjivani corona ghar seva (service provided by government for home quarantine people), RTPCR swab collection, and posting in wards intensive care units (ICUs). While executing duties, had a significant degree of ambiguity apprehension. The purpose this study was to dete...

Antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the presence seed transmissibility of bean common mosaic virus-blackeye cowpea (BCMV-BICM) in farm- retained lots obtained from 46 locations, including markets farms major growing areas Ashanti Brong Ahafo regions Ghana. In growout tests, virus symp...

Abstract Background Corona virus disease has been associated with a wide variety of fungal and bacterial co-infections. These secondary infections could be due to the irrational use antibiotics, immunosuppressive therapy, pre-existing co morbidities, immune modulator effects virus. But here, we report very rare occurring rhino-orbito-cerebral invasive sinusitis in malnourished child no other mo...