Previous studies of cat visual cortex have shown that the spatiotemporal (S-T) structure of simple cell receptive fields correlates with direction selectivity. However, great heterogeneity exists in the relationship and this has implications for models. Here we report a laminar basis for some of the heterogeneity. S-T structure and direction selectivity were measured in 101 cells using stationa...

The DEVS framework of discrete event modeling is known to be compatible with the object-oriented (OO) world view. This paper proposes a methodology for the OO development of war game models within the framework. The methodology develops war game models at two layers: a discrete event system (DES) layer and an object model (OM) layer. Within the methodology the DES layer represents abstract beha...

Sinusoidal patterns of spatially modulated near-infrared 650 nm structured light are used to interrogate multilayer phantoms and tissue. Diffuse reflectance is imaged at multiple spatial frequencies from 0–0.3 mm−1. ac and dc components of the image are fit to a two layer model formulated from the diffusion approximation to the Boltzman transport equation. The two-layer model depends on optical...

The S-layer of Bacillus stearothermophilus PV72/p2 shows oblique lattice symmetry and is composed of identical protein subunits with a molecular weight of 97,000. The isolated S-layer subunits could bind and recrystallize into the oblique lattice on native peptidoglycan-containing sacculi which consist of peptidoglycan of the A1gamma chemotype and a secondary cell wall polymer with an estimated...

The recombinant bacterial surface layer (S-layer) protein rSbpA of Lysinibacillus sphaericus CCM 2177 is an ideal model system to study non-classical nucleation and growth of protein crystals at surfaces since the recrystallization process may be separated into two distinct steps: (i) adsorption of S-layer protein monomers on silicon surfaces is completed within 5 min and the amount of bound S-...

Corresponding to the sole or basic component of the surface (S)-layer surrounding the archaeal cell in most known cases, S-layer glycoproteins are in direct contact with the harsh environments that characterize niches where Archaea can thrive. Accordingly, early work examining archaeal S-layer glycoproteins focused on identifying those properties that allow members of this group of proteins to ...

Bacterial surface layers (S-layers) are crystalline arrays of self-assembling proteinaceous subunits called S-layer proteins (Slps) that comprise the outermost layer of the cell envelope. Many additional proteins that are associated with or embedded within the S-layer have been identified in Lactobacillus acidophilus NCFM, an S-layer-forming bacterium that is widely used in fermented dairy prod...

The S-layer glycoprotein is the sole component of the protein shell surrounding Haloferax volcanii cells. The deduced amino acid sequence of the S-layer glycoprotein predicts the presence of a C-terminal membrane-spanning domain. However, several earlier observations, including the ability of EDTA to selectively solubilize the protein, are inconsistent with the presence of a trans-membrane sequ...

Bacillus anthracis grows in chains of rod-shaped cells, a trait that contributes to its escape from phagocytic clearance in host tissues. Using a genetic approach to search for determinants of B. anthracis chain length, we identified mutants with insertional lesions in secA2. All isolated secA2 mutants exhibited an exaggerated chain length, whereas the dimensions of individual cells were not ch...

Existence of gas-hydrates in the marine sediments elevates the velocity of seismic waves, whereas even a small amount of underlying free-gas decreases the seismic velocity considerably. These changes in velocities can be used for the assessment of gas-hydrates. The traveltime inversion is an effective method to derive a 2-D velocity structure from the seismic data. We have applied the method to...