We identified and sequenced a segment of Chlamydia trachomatis chromosomal DNA that shows homology to the Escherichia coli spc and distal region of the S10 ribosomal protein (r-protein) operons. Its sequence revealed a high degree of nucleotide and operon context conservation with the E. coli r-protein genes. The C. trachomatis spec operon contains the r-protein genes for L14, L24, L5, S8, L6, ...

The human/sheep xenograft model has proven valuable in assessing the in vivo hematopoietic activity of stem cells from a variety of fetal and postnatal human sources. CD34 /lineage or CD34 /CD38 cells isolated from human embryonic stem cells (hESCs) differentiated on S17 feeder layer were transplanted by intraperitoneal injections into fetal sheep. Chimerism in primary transplants was establish...

Here we present the generation and function of two sets of bacterial plasmids that harbor fluorescent genes encoding either blue, cyan, yellow or red fluorescent proteins. In the first set, protein expression is controlled by the strong and constitutive nptII promoter whereas in the second set, the strong tac promoter was chosen that underlies LacI(q) regulation. Furthermore, the plasmids are m...

A cell culture system consisting of mouse S17 stromal cells supplemented with cytokines was developed for hematopoietic differentiation of rhesus monkey embryonic stem (ES) cells. The differentiated colonies that formed contained clusters of hematopoietic-like cells, as well as structures similar in appearance to embryonic blood islands. When this culture system was supplemented with bone morph...

The crystal structure of a specimen of `Pb-rich' chabournéite from Jas Roux, Hautes-Alpes, France, with the chemical formula obtained by electron microprobe analysis of Ag(0.04 (1))Tl(2.15 (2))Pb(0.64 (1))Sb(5.12 (1))As(5.05 (1))S(17.32 (5)), has been solved by X-ray single-crystal diffraction on the basis of 36,550 observed reflections (with F(o) > 4σF(o)) with a final R1 = 0.074. Pb-rich chab...

PURPOSE To determine the relative dose rate distribution around the new (125)I brachytherapy source IsoSeed I25.S17plus and report results in a form suitable for clinical use. Results for the new source are also compared to corresponding results for other commercially available (125)I sources of similar design. MATERIAL AND METHODS Monte Carlo simulations were performed using the MCNP5 v.1.6 ...

ISPst9 is an ISL3-like insertion sequence (IS) that was recently described in the naphthalene-degrading organism Pseudomonas stutzeri strain AN10. In this paper we describe a novel strong IS regulation stimulus; transposition of ISPst9 is induced in all P. stutzeri AN10 cells after conjugative interaction with Escherichia coli. Thus, we observed that in all P. stutzeri AN10 cells that received ...

Since identification of the CFTR gene over 25 years ago, gene therapy for cystic fibrosis (CF) has been actively developed. More recently gene therapy has been joined by other forms of "genetic medicines" including mRNA delivery, as well as genome editing and mRNA repair-based strategies. Proof-of-concept that gene therapy can stabilize the progression of CF lung disease has recently been estab...

GeV beams of light ions and electrons are used for creating a high flux of real and virtual photons, with which some problems in Nuclear Astrophysics are studied. GeV B beams are used to study the Coulomb dissociation of B and thus the Be(p, γ)B reaction. This reaction is one of the major source of uncertainties in estimating the B solar neutrino flux and a critical input for calculating the B ...

Supplemental
Section
S1
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Genome
Sequencing
and
Assembly........................................... 2
 Supplemental
Section
S2
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Indel
Assessment
With
the
Neutral
Indel
Model ................11
 Supplemental
Section
S3
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Great
Ape
Divergence
Estimate
via
WGS
Read
Mapping..11
 Supplemental
Section
S4
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Short
Read
Sequencing................................................................13
 Suppl...