Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating saccharides. Oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and the reductive amination reactions were essentially complete after approximately 16 h under the given experimental conditions. Saccharide-ANTS adducts were then separated...

A novel fluorescence detector for high-performance liquid chromatography has been designed to minimize the instrumental limitations of conventional flow-through, fluorometers. This unit contributes less than 5 mul to the "dead volume" of the chromatographic system. Sensitivity comparable to that of existing flow-through fluorometers has been attained without optimizing instrumental parameters. ...

teins on the tumor cells was shown to be specific, pH de pendent, and concentration dependent. Native ricin, abrin, D-galactose, and its sterically related saccharides inhibit this specific binding, while concanavalin A, bovine serum albumin, heat-denatured abrin or ricin, and other sac charides do not. In the presence of D-galactose, the inhibi tion of protein biosynthesis by ricin does not oc...

This article gives an overview of uncommon replaceable matrices (gels) for capillary gel electrophoresis. This electrophoretic technique is useful mainly for the separation and analysis of biopolymers-nucleic acids and their fragments, and proteins/peptides. Commonly used gels are not reviewed. Those mentioned and discussed here are gels containing saccharides, newly developed acrylamide-based ...

Stage-specific differences in wheat germ agglutinin (WGA) binding saccharides were demonstrated between the surfaces of the eggs, L1 larvae, young aduhs, and old adults of Caenorhabditis elegans. The WGA binding was to n-acetylglucosamine groups but not to terminally linked n-acetylneuraminic acids. An age-related decrease in WGA binding occurred in adults, supporting previous findings of a dec...

The C5''-OH group in neomycin B was glycosylated with a variety of mono- and di-saccharides to probe the effect of introduction of additional binding elements on antibacterial activity and interaction with the aminoglycosides modifying enzyme APH(3')-IIIa. The designed structures show antibacterial activity superior to that of neomycin B against pathogenic and resistant strains, while in parall...

Conditions have been established for the rapid and efficient conjugation of reducing oligosaccharides (di- to deca-saccharides) to dipalmitoyl phosphatidylethanolamine. The resulting neoglycolipids derived from several naturally occurring oligosaccharides and a series of N-linked high-mannose-type oligosaccharides released by hydrazinolysis from RNAase B showed specific and potent reactivities,...

A new class of surface-active compounds based on the combination of silicones and boronic acids is described. The properties of the compounds can be tuned by manipulation of both the hydrophobic (silicone size and 3D structure) and hydrophilic components (by binding different saccharides to the boronic acid). Stabilization of the four-coordinate boron structure is provided by Tris buffer that a...