Highlights • Reconstruction of mitochondrial protein import and cofactor metabolism in the model eukaryote Saccharomyces cerevisiae Quantification proton motive force shows significant energy cost metabolite transport Protein activity depends on growth rate respiratory iron-sulfur cluster requirements show dependence

The present study analyzes the lack of culturability of different non-Saccharomyces strains due to interaction with Saccharomyces cerevisiae during alcoholic fermentation. Interaction was followed in mixed fermentations with 1:1 inoculation of S. cerevisiae and ten non-Saccharomyces strains. Starmerella bacillaris, and Torulaspora delbrueckii indicated longer coexistence in mixed fermentations ...

OBJECTIVE To compare the efficacy of Saccharomyces boulardii and lactic acid producing probiotics in addition to usual treatment regimen to cure diarrhea among children (6 months to 5 years of age). STUDY DESIGN Randomized controlled trial. PLACE AND DURATION OF STUDY Department of Pediatrics, Sheikh Zayed Hospital, Lahore, from February to July 2015. METHODOLOGY Children suffering from a...

The popularity of non-alcoholic beers has been increasing over the past few years. Maltose-negative strains different genera are frequently used to obtain low alcohol content. S. cerevisiae hybrids with other Saccharomyces species offer interesting inherited flavour characteristics; however, their use in beer production is rare. In this work, we constructed six maltose-negative parental (modifi...

The lager brewing yeasts, Saccharomyces pastorianus (synonym Saccharomyces carlsbergensis), are allopolyploid, containing parts of two divergent genomes. Saccharomyces cerevisiae contributed to the formation of these hybrids, although the identity of the other species is still unclear. The presence of alleles specific to S. cerevisiae and S. pastorianus was tested for by PCR/RFLP in brewing yea...

BACKGROUND Lignocellulosic biomass is a common resource across the globe, and its fermentation offers a promising option for generating renewable liquid transportation fuels. The deconstruction of lignocellulosic biomass releases sugars that can be fermented by microbes, but these processes also produce fermentation inhibitors, such as aromatic acids and aldehydes. Several research projects hav...