نتایج جستجو برای: sandwich method
تعداد نتایج: 1638944 فیلتر نتایج به سال:
We study delamination in a sandwich panel due to transient finite plane strain elastic deformations caused by local water slamming loads and use the boundary element method to analyze motion of water and the finite element method to determine deformations of the panel. The cohesive zone model is used to study delamination initiation and propagation. The fluid is assumed to be incompressible and...
OBJECTIVE To prepare monoclonal antibodies (MAb) and antisera specific for Escherichia coli (E. coli) O157, and to develop a sandwich enzyme-linked immunosorbent assay (ELISA) to detect E. coli O157 in foods. METHODS Spleen cells from BALB/c mice immunized with the somatic antigen of E. coli O157:H7 were fused with murine Sp2/0 myeloma cells. The hybridoma cell line specific for E. coli O157 ...
The objective of this work is to study the large deflection of geometrically asymmetric metal foam core sandwich beam under transverse loading by a fiat punch. A yield criterion is proposed for geometrically asymmetric metal foam core sandwich structures, and then analytical solution for the large deflection of a fully clamped slender sandwich beam is obtained, in which the interaction of bendi...
OBJECTIVE To establish a sandwich ELISA method for detecting vascular endothelial growth factor (VEGF) in sera of population and the patients with hepatocellular carcinoma (HCC). METHODS Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF pro...
the aim of this study was to determine the microleakage at the cervical margin of the class II composite resin syntac with and without a cervical amalgam base and to compare microleakage restoration at the occlusal margins of restorations and inferface of amalgam and composite.68 extracted sound permanent premolar were used.68 class II slot cavities were prepared in these teth.the teeth were ra...
We have developed a new method for quantifying teicoplanin in complex matrixes, a receptor-antibody sandwich assay (RASA). The method is based on bioselective adsorption of teicoplanin onto microtiter plates coated with albumin-epsilon-aminocaproyl-D-alanyl-D-alanine, a synthetic analog of its biological target, and reaction with anti-teicoplanin antibodies. The sandwich complexes are detected ...
This study presents a free vibration analysis of asymmetric sandwich structures comparatively. Sandwich were manufactured by the hand-layup vacuum bagging method. Symmetric and analyzed experimentally to evaluate effect asymmetry on characteristic structures. Free was performed using VIBXPERT II under clamped-free boundary conditions. The frequency response function (FRF) is obtained from modal...
BACKGROUND We describe a method for specific, quantitative and quick detection of human collagen prolyl 4-hydroxylase (C-P4H), the key enzyme for collagen prolyl-4 hydroxylation, in crude samples based on a sandwich ELISA principle. The method is relevant to active C-P4H level monitoring during recombinant C-P4H and collagen production in different expression systems. The assay proves to be spe...
panels with a genetic algorithm, Applied Acoustics, Volume 70, Issue 3, March 2009, Pages 416-425, ISSN 0003-682X, http://dx.doi.org/10.1016/j.apacoust.2008.06.003. 1 Optimal design of acoustical sandwich panels with a genetic algorithm Tongan Wang, Shan Li, Steven R. Nutt 1. University of Southern California, 3651 Watts Way, VHE 602, Los Angeles, CA 90089, United States Abstract: An optimizati...
Loss of Sandwich Panels by Statistical Energy Analysis Approach. ASME. J. Vib. Acoust.2010;132(1):011004-011004-7. doi:10.1115/1.4000459. Predicting the Sound Transmission Loss of Sandwich Panels by Statistical Energy Analysis Approach Tongan Wang, Shan Li, Shankar Rajaram, Steven R. Nutt 1. University of Southern California, 3651 Watt Way, VHE 602, Los Angeles, CA 90089 Abstract: A statistical...
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