OBJECTIVE To prepare monoclonal antibodies (MAb) and antisera specific for Escherichia coli (E. coli) O157, and to develop a sandwich enzyme-linked immunosorbent assay (ELISA) to detect E. coli O157 in foods. METHODS Spleen cells from BALB/c mice immunized with the somatic antigen of E. coli O157:H7 were fused with murine Sp2/0 myeloma cells. The hybridoma cell line specific for E. coli O157 ...

OBJECTIVE To establish a sandwich ELISA method for detecting vascular endothelial growth factor (VEGF) in sera of population and the patients with hepatocellular carcinoma (HCC). METHODS Full length and two truncated human VEGF cDNA sequences were amplified from a commercial plasmid pBLAST49-hVEGF by PCR and inserted into the prokaryotic-expression plasmid pET-32a or pGEX-2T. Various VEGF pro...

An orthographically similar masked nonword prime facilitates responding in a lexical decision task (Forster & Davis, 1984). Recently, this masked priming paradigm has been used to evaluate models of orthographic coding--odels that attempt to quantify prime-target similarity. One general finding is that priming effects often do not occur when prime-target similarity is moderate, a result that th...

A series of analytical and experimental investigations is presented to study the response and failure of pyramidal truss core sandwich panels made of carbon fiber composite under axial compression. In the analytical part of the study, three failure modes: (i) Euler or core shear macro-buckling, (ii) face wrinkling, and (iii) face sheet crushing, were considered and theoretical relationships for...

In a Z-core sandwich panel, the shear stiffness of the sandwich panel in the weak direction which is perpendicular to the placement of the Z-cores is much smaller than the bending stiffness because the hollow section between the two facing plates cannot sustain shear action. Because of this fact, the shear deformation of a Z-core sandwich panel under bending in the weak direction cannot be igno...

Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g s...

BACKGROUND Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic t...

The main aim of this paper is to investigate bending behavior in sandwich plates with functionally graded carbon nanotube reinforced composite (FG-CNTRC) face sheets with considering the effects of carbon nanotube (CNT) aggregation. The sandwich plates are assumed resting on Winkler-Pasternak elastic foundation and a mesh-free method based on first order shear deformation theory (FSDT) is devel...

Sandwich panel with central core honeycomb aluminum as one of the main shielding structures because of their high strength-to-weight ratio and improved energy absorption capacity, has been attracted the attention of many researchers and practitioners in recent years. This paper initiates and presents a new type of central core honeycomb formed by hydroforming process. The study is augmented by ...

In this paper, a new roofing system is introduced, which is constructued using the precast composite sandwich panels. This roofing sandwich panels system consists of three kinds of precast concrete sandwich panels including capital panels, beam or between columns panels, and slab or middle panels. The panels are composed of three layers; A high strength reinforced concrete top layer. A thick la...