نتایج جستجو برای: sars cov 2

تعداد نتایج: 2534203  

Journal: :Clinical chemistry 2003
Enders K O Ng David S Hui K C Allen Chan Emily C W Hung Rossa W K Chiu Nelson Lee Alan Wu Stephen S C Chim Yu K Tong Joseph J Y Sung John S Tam Y M Dennis Lo

BACKGROUND The availability of an early diagnostic tool for severe acute respiratory syndrome (SARS) would have major public health implications. We investigated whether the SARS coronavirus (SARS-CoV) can be detected in serum and plasma samples during the early stages of SARS and studied the potential prognostic implications of such an approach. METHODS We developed two real-time quantitativ...

Journal: :Journal of virology 2008
Bing Zhou Junli Liu Qiuna Wang Xuan Liu Xiaorong Li Ping Li Qingjun Ma Cheng Cao

Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N) protein of SARS-CoV as a bait, the C terminus (amino acids 251 to 422) of the N protein was found to interact with human elongation factor 1-alpha (EF1alpha), an essential component of the tr...

2011
I-Chueh Huang Charles C. Bailey Jessica L. Weyer Sheli R. Radoshitzky Michelle M. Becker Jessica J. Chiang Abraham L. Brass Asim A. Ahmed Xiaoli Chi Lian Dong Lindsay E. Longobardi Dutch Boltz Jens H. Kuhn Stephen J. Elledge Sina Bavari Mark R. Denison Hyeryun Choe Michael Farzan

Interferon-inducible transmembrane proteins 1, 2, and 3 (IFITM1, 2, and 3) are recently identified viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Here we show that IFITM proteins restricted infection mediated by the entry glycoproteins (GP(1,2)) of Marburg and Ebola filoviruses (MARV, EBOV). Consistent with these observations...

Journal: :Japanese journal of infectious diseases 2005
Kazuo Ohnishi Masahiro Sakaguchi Tomohiro Kaji Kiyoko Akagawa Tadayoshi Taniyama Masataka Kasai Yasuko Tsunetsugu-Yokota Masamichi Oshima Kiichi Yamamoto Naomi Takasuka Shu-ichi Hashimoto Manabu Ato Hideki Fujii Yoshimasa Takahashi Shigeru Morikawa Koji Ishii Tetsutaro Sata Hirotaka Takagi Shigeyuki Itamura Takato Odagiri Tatsuo Miyamura Ichiro Kurane Masato Tashiro Takeshi Kurata Hiroshi Yoshikura Toshitada Takemori

In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secr...

Journal: :The Journal of biological chemistry 2012
Kailang Wu Guiqing Peng Matthew Wilken Robert J Geraghty Fang Li

The severe acute respiratory syndrome coronavirus (SARS-CoV) from palm civets has twice evolved the capacity to infect humans by gaining binding affinity for human receptor angiotensin-converting enzyme 2 (ACE2). Numerous mutations have been identified in the receptor-binding domain (RBD) of different SARS-CoV strains isolated from humans or civets. Why these mutations were naturally selected o...

Journal: :Clinical and diagnostic laboratory immunology 2004
Yee-Joo Tan Phuay-Yee Goh Burtram C Fielding Shuo Shen Chih-Fong Chou Jian-Lin Fu Hoe Nam Leong Yee Sin Leo Eng Eong Ooi Ai Ee Ling Seng Gee Lim Wanjin Hong

A new coronavirus (severe acute respiratory syndrome coronavirus [SARS-CoV]) has been identified to be the etiological agent of severe acute respiratory syndrome. Given the highly contagious and acute nature of the disease, there is an urgent need for the development of diagnostic assays that can detect SARS-CoV infection. For determination of which of the viral proteins encoded by the SARS-CoV...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2004
Jianhua Sui Wenhui Li Akikazu Murakami Azaibi Tamin Leslie J Matthews Swee Kee Wong Michael J Moore Aimee St Clair Tallarico Mobolaji Olurinde Hyeryun Choe Larry J Anderson William J Bellini Michael Farzan Wayne A Marasco

Effective prophylaxis and antiviral therapies are urgently needed in the event of reemergence of the highly contagious and often fatal severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) infection. We have identified eight recombinant human single-chain variable region fragments (scFvs) against the S1 domain of spike (S) protein of the SARS-CoV from two nonimmune human antibody libr...

2018
Maria L. Agostini Erica L. Andres Amy C. Sims Rachel L. Graham Timothy P. Sheahan Xiaotao Lu Everett Clinton Smith James Brett Case Joy Y. Feng Robert Jordan Adrian S. Ray Tomas Cihlar Dustin Siegel Richard L. Mackman Michael O. Clarke Ralph S. Baric Mark R. Denison

Emerging coronaviruses (CoVs) cause severe disease in humans, but no approved therapeutics are available. The CoV nsp14 exoribonuclease (ExoN) has complicated development of antiviral nucleosides due to its proofreading activity. We recently reported that the nucleoside analogue GS-5734 (remdesivir) potently inhibits human and zoonotic CoVs in vitro and in a severe acute respiratory syndrome co...

2008
Alison C. Mathewson Alexandra Bishop Yongxiu Yao Fred Kemp Junyuan Ren Hongying Chen Xiaodong Xu Ben Berkhout Lia van der Hoek Ian M. Jones

Although in different groups, the coronaviruses severe acute respiratory syndrome-coronavirus (SARS-CoV) and NL63 use the same receptor, angiotensin converting enzyme (ACE)-2, for entry into the host cell. Despite this common receptor, the consequence of entry is very different; severe respiratory distress in the case of SARS-CoV but frequently only a mild respiratory infection for NL63. Using ...

Journal: :Journal of virological methods 2004
Miriam E R Darnell Kanta Subbarao Stephen M Feinstone Deborah R Taylor

Severe acute respiratory syndrome (SARS) is a life-threatening disease caused by a novel coronavirus termed SARS-CoV. Due to the severity of this disease, the World Health Organization (WHO) recommends that manipulation of active viral cultures of SARS-CoV be performed in containment laboratories at biosafety level 3 (BSL3). The virus was inactivated by ultraviolet light (UV) at 254 nm, heat tr...

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